Mnsod1 促进糙皮侧耳的生长发育，并增强菌丝体对热应激的耐受性。

Mnsod1 promotes the development of Pleurotus ostreatus and enhances the tolerance of mycelia to heat stress.

机构信息

College of Food Science and Engineering, Shanxi Agricultural University, 1 Mingxian South Road, Taigu, 030801, Shanxi, China.

Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.

出版信息

Microb Cell Fact. 2022 Aug 8;21(1):155. doi: 10.1186/s12934-022-01878-2.

DOI:10.1186/s12934-022-01878-2

PMID:35934720

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9358896/

Abstract

BACKGROUND

Superoxide dismutases (SODs, EC 1.15.1.1) are defense proteins that can be used as sweepers to clear reactive oxygen species (ROS). They have been widely studied in the plant. Intensive research demonstrates that SOD plays an essential role in plants. However, in Pleurotus ostreatus, the function and regulatory pathway of SOD in the growth and development and the abiotic stress response have not been clear.

RESULTS

In this study, three MnSOD-encoding genes of the P. ostreatus CCMSSC00389 strain were cloned and identified. Mnsod1, Mnsod2, and Mnsod3 were interrupted by 3, 7, and 2 introns, and encoded proteins of 204, 220, and 344 amino acids, respectively. By comparing the relative expression of three MnSOD-encoding genes in mycelia, the results showed that the gene with the highest primary expression was Mnsod1. Subsequently, the function of P. ostreatus Mnsod1 was explored by overexpression (OE) and RNA interference (RNAi). The results showed that during the growth and development of P. ostreatus, MnSOD1 protein increased gradually from mycelia to the fruiting body, but decreased in spores. The change of Mnsod1 transcription level was not consistent with the changing trend of MnSOD1 protein. Further studies showed that during primordia formation, the expression of Mnsod1 gradually increased, reaching a peak at 48 h, and the transcription level was 2.05-folds compared to control. HO content progressively accumulated during the formation of primordia, and its change trend was similar to that of Mnsod1 transcription. OE-Mnsod1-1 and OE-Mnsod1-21 strains accelerated the formation of primordia. The results suggested that Mnsod1 may participate in the formation rate of P. ostreatus primordium by regulating the signal molecule HO. In addition, OE-Mnsod1-1 and OE-Mnsod1-21 strains shortened the mycelial recovery time after heat stress and improved the tolerance of the strains to 2.5 mM and 5 mM HO, which showed that Mnsod1 was involved in the response of P. ostreatus mycelium to heat stress.

CONCLUSIONS

This study indicates that Mnsod1 plays an active role in the formation of P. ostreatus primordia and the response to abiotic stress.

摘要

背景

超氧化物歧化酶（SODs，EC 1.15.1.1）是防御蛋白，可以用作清除活性氧（ROS）的清道夫。它们在植物中得到了广泛的研究。大量研究表明，SOD 在植物中起着至关重要的作用。然而，在糙皮侧耳（Pleurotus ostreatus）中，SOD 在生长发育和非生物胁迫响应中的功能和调控途径尚不清楚。

结果

本研究克隆并鉴定了糙皮侧耳 CCMSSC00389 菌株的三个 MnSOD 编码基因。Mnsod1、Mnsod2 和 Mnsod3 分别被 3、7 和 2 个内含子打断，分别编码 204、220 和 344 个氨基酸的蛋白质。通过比较三个 MnSOD 编码基因在菌丝中的相对表达，结果表明初级表达最高的基因是 Mnsod1。随后，通过过表达（OE）和 RNA 干扰（RNAi）探索糙皮侧耳 Mnsod1 的功能。结果表明，在糙皮侧耳的生长发育过程中，MnSOD1 蛋白从菌丝体逐渐增加到子实体，但在孢子中减少。Mnsod1 转录水平的变化与 MnSOD1 蛋白的变化趋势不一致。进一步的研究表明，在原基形成过程中，Mnsod1 的表达逐渐增加，在 48 小时达到峰值，转录水平比对照高 2.05 倍。HO 含量在原基形成过程中逐渐积累，其变化趋势与 Mnsod1 转录相似。OE-Mnsod1-1 和 OE-Mnsod1-21 菌株加速了原基的形成。结果表明，Mnsod1 可能通过调节信号分子 HO 参与调控糙皮侧耳原基的形成速度。此外，OE-Mnsod1-1 和 OE-Mnsod1-21 菌株缩短了热应激后菌丝体的恢复时间，并提高了菌株对 2.5mM 和 5mM HO 的耐受性，表明 Mnsod1 参与了糙皮侧耳菌丝体对热应激的响应。