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基于计算机模拟对……中超氧化物歧化酶基因家族的鉴定与表征

Insilco identification and characterization of superoxide dismutase gene family in .

作者信息

Iqbal Qureshi Asif M, Sofi Mehraj Uddin, Dar N A, Khan M H, Mahdi S S, Dar Zahoor A, Bangroo Shabir, El-Serehy Hamed A, Hefft Daniel Ingo, Popescu Simona Mariana

机构信息

ARSSSS, Pampore, Sher-e-Kashmir University of Agricultural Sciences and Technology Shalimar Kashmir, India.

HMAARI, Leh, Sher-e-Kashmir University of Agricultural Sciences and Technology Shalimar Kashmir, India.

出版信息

Saudi J Biol Sci. 2021 Oct;28(10):5526-5537. doi: 10.1016/j.sjbs.2021.08.009. Epub 2021 Aug 8.

Abstract

Superoxide Dismutase are defense associated proteins that detoxify ROS and primarily serve as scavengers. They have been described in numerous plant species, but their in-depth characterization in has not been reported. Therefore, the present investigation on genome wide study of gene family was conducted to identify Br genes, their domain-based organization, gene structure analysis, phylogenetic analysis, intron-exon structure of genes and expression analysis. The sequence characterization of Super oxide dismutase gene family in , their syntenic associateship of conserved motifs and phylogenetic correlationship, prediction of -elements and determing the expression analysis in distinct tissues namely plant callus, root, stem, leaf, flower, and silique under abiotic conditions have been analysed using different software's. The study on gene family identified 17 Br genes which were grouped into eight Br and nine Br domain-based organization. Furthermore, the conserved character of Br were confirmed by intron-exon organisation, motif arrangements and domain architectural investigations. Expression analysis using RNA Sequence data of different developmental stages proclaimed that genes were manifested in all six tissues with an exception of Br which was not manifested in roots; however, whose transcript was detected in all other tested tissues. The study has genome wide insight into the occurrence and functional specifications of Br gene family in that can be potentially utilized in breeding program for resilience to climate change and abiotic stresses tolerance variety.

摘要

超氧化物歧化酶是与防御相关的蛋白质,可清除活性氧,主要起清除剂的作用。它们已在许多植物物种中被描述,但在[具体物种]中尚未有对其深入的特征描述。因此,本研究对[具体物种]基因家族进行全基因组研究,以鉴定Br基因、其基于结构域的组织、基因结构分析、系统发育分析、基因的内含子-外显子结构以及表达分析。利用不同软件分析了[具体物种]中超氧化物歧化酶基因家族的序列特征、保守基序的共线性关联和系统发育相关性、-元件预测以及在非生物条件下植物愈伤组织、根、茎、叶、花和角果等不同组织中的表达分析。对[具体物种]基因家族的研究鉴定出17个Br基因,这些基因被分为基于八个Br和九个Br结构域的组织。此外,通过内含子-外显子组织、基序排列和结构域结构研究证实了Br的保守特征。使用不同发育阶段的RNA序列数据进行的表达分析表明,除了Br在根中不表达外,所有基因在所有六种组织中均有表达;然而,其转录本在所有其他测试组织中均被检测到。该研究对[具体物种]中Br基因家族的发生和功能特性有全基因组的深入了解,可潜在地用于育种计划,以培育适应气候变化和耐受非生物胁迫的品种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ce1/8459115/b922ffa11683/gr1.jpg

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