Ghoreishi Fatemeh Sadat, Roghanian Rasoul, Emtiazi Giti
Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Adv Pharm Bull. 2022 May;12(3):424-436. doi: 10.34172/apb.2022.047. Epub 2021 Mar 27.
Chronic wounds have made a challenge in medical healthcare due to their biofilm infections, which reduce the penetrance of the antibacterial agents in the injury site. In infected wounds, the most common bacterial strains are and . Biofilm disruption in chronic wounds is crucial in wound healing. Due to their broad-spectrum antibacterial properties and fewer side effects, anti-biofilm peptides, especially bacteriocins, are promising in the healing of chronic wounds by biofilm destruction. This study reviews the effects of antimicrobial and anti-biofilm agents, including bacteriocins and protease enzymes as a novel approach, on wound healing, along with analyzing the molecular docking between a bacterial protease and biofilm components. Among a large number of anti-biofilm bacteriocins identified up to now, seven types have been registered in the antimicrobial peptides (AMPs) database. Although it is believed that bacterial proteases are harmful in wound healing, it has recently been demonstrated that these proteases like the human serine protease, in combination with AMPs, can improve wound healing by biofilm destruction. In this work, docking results between metalloprotease from and proteins of and involved in biofilm production, showed that this bacterial protease could efficiently interact with biofilm components. Infected wound healing is an important challenge in clinical trials due to biofilm production by bacterial pathogens. Therefore, simultaneous use of proteases or anti-biofilm peptides with antimicrobial agents could be a promising method for chronic wound healing.
慢性伤口因其生物膜感染给医疗保健带来了挑战，生物膜感染会降低抗菌剂在伤口部位的渗透率。在感染伤口中，最常见的细菌菌株是……和……。慢性伤口中的生物膜破坏对伤口愈合至关重要。由于其广谱抗菌特性和较少的副作用，抗生物膜肽，尤其是细菌素，在通过破坏生物膜促进慢性伤口愈合方面很有前景。本研究综述了抗菌和抗生物膜剂，包括细菌素和蛋白酶作为一种新方法，对伤口愈合的影响，同时分析了一种细菌蛋白酶与生物膜成分之间的分子对接。在迄今鉴定出的大量抗生物膜细菌素中，有七种已在抗菌肽（AMPs）数据库中注册。尽管人们认为细菌蛋白酶在伤口愈合中是有害的，但最近已证明，这些蛋白酶如人丝氨酸蛋白酶，与AMPs联合使用时，可通过破坏生物膜来改善伤口愈合。在这项工作中，来自……的金属蛋白酶与参与生物膜产生的……和……的蛋白质之间的对接结果表明，这种细菌蛋白酶可以有效地与生物膜成分相互作用。由于细菌病原体产生生物膜，感染伤口愈合在临床试验中是一项重要挑战。因此，同时使用蛋白酶或抗生物膜肽与抗菌剂可能是慢性伤口愈合的一种有前景的方法。
