Rackus Darius G, Jusková Petra, Yokoyama Fumiaki, Dittrich Petra S
Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel, Switzerland.
Biomicrofluidics. 2022 Aug 1;16(4):044105. doi: 10.1063/5.0091704. eCollection 2022 Jul.
Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping and cultivating bacteria in hydrogel plugs. Ring-shaped isolation valves are used to define the shape of the plugs and also to control exposure of the plugs to the surrounding medium. We demonstrate bacterial cultivation, determination of the minimum inhibitory concentration of an antibiotic, and transient dosing of an antibiotic at sub-1-h doses. The transient dosing experiments reveal that at dose durations on the order of minutes, ampicillin's bactericidal effect has both a time and concentration dependency.
微流控工具非常适合用于研究细菌，因为它们能够对小菌落或单个细胞进行分析。然而，目前用于研究细菌对抗生素反应的技术在很大程度上仅限于静态给药。在此，我们描述了一种微流控装置以及一种在水凝胶塞中捕获和培养细菌的方法。环形隔离阀用于确定塞子的形状，还用于控制塞子与周围培养基的接触。我们展示了细菌培养、抗生素最低抑菌浓度的测定以及亚1小时剂量的抗生素瞬时给药。瞬时给药实验表明，在几分钟量级的给药持续时间下，氨苄青霉素的杀菌作用具有时间和浓度依赖性。
