来自临床菌株的接合型KpVP-2型毒力质粒的遗传与功能特性分析
Genetic and Functional Characterization of a Conjugative KpVP-2-Type Virulence Plasmid From a Clinical Strain.
作者信息
Yang Xuemei, Liu Xiaoxuan, Xu Yating, Yang Chen, Chan Edward Wai-Chi, Shum Hoi-Ping, Chen Sheng
机构信息
Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
State Key Lab of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China.
出版信息
Front Microbiol. 2022 Jul 22;13:914884. doi: 10.3389/fmicb.2022.914884. eCollection 2022.
The main mechanism of virulence in is the acquisition of virulence plasmids (KpVPs), which include two dominant types, namely, KpVP-1 (carrying 1, 1, , and ) and KpVP-2 (carrying 2, 2, and ). Both are non-conjugative and associated with different hypervirulent clones. In contrast to KpVP-1 reported in K1, K2, and other serotypes of , KpVP-2 was only reported in K2 strains and rarely characterized. In this study, we identified a conjugative KpVP-2-type virulence plasmid from a clinical hypervirulent strain. This plasmid was generated by the integration of conjugative transfer genes into the KpVP-2-type plasmid Kp52.145 II and could be readily conjugated to strain EC600 and strains of various types which are clinically existing, mediating hypervirulence. Furthermore, this kind of conjugative KpVP-2-type virulence plasmid has been disseminated in clinical settings in Hong Kong and other regions of the world. The generation of conjugative virulence plasmid may promote its transmission and explain the evolution of this type of virulence plasmid.
的主要毒力机制是获得毒力质粒（KpVPs），其包括两种主要类型，即KpVP - 1（携带1、1、 和 ）和KpVP - 2（携带2、2和 ）。两者均为非接合型，且与不同的高毒力克隆相关。与在K1、K2和其他血清型的 中报道的KpVP - 1不同，KpVP - 2仅在K2菌株中报道过，且很少被鉴定。在本研究中，我们从一株临床高毒力 菌株中鉴定出一种接合型KpVP - 2型毒力质粒。该质粒是通过将接合转移基因整合到KpVP - 2型质粒Kp52.145 II中产生的，并且可以很容易地与菌株EC600以及临床上存在的各种类型的 菌株进行接合，介导高毒力。此外，这种接合型KpVP - 2型毒力质粒已在香港和世界其他地区的临床环境中传播。接合型毒力质粒的产生可能促进其传播，并解释了这种类型毒力质粒的进化。
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