Klaper Kathleen, Wendt Sebastian, Lübbert Christoph, Lippmann Norman, Pfeifer Yvonne, Werner Guido
Division Nosocomial Pathogens and Antibiotic Resistance, Department of Infectious Diseases, Robert Koch Institute, Wernigerode Branch, 38855 Wernigerode, Germany.
Division of Infectious Diseases and Tropical Medicine, Department of Medicine II, Leipzig University Hospital, 04109 Leipzig, Germany.
Microorganisms. 2021 Jan 8;9(1):133. doi: 10.3390/microorganisms9010133.
Hypervirulent (hvKp) is a novel pathotype that has been rarely described in Europe. This study characterizes a hvKp isolate that caused a community-acquired infection. The hypermucoviscous () strain 18-0005 was obtained from a German patient with tonsillopharyngitis in 2017. Antibiotic susceptibility testing was performed and the genome was sequenced by Illumina and Nanopore technology. Whole genome data were analyzed by conducting core genome multilocus sequence typing (cgMLST) and single nucleotide polymorphism (SNP) analysis. Virulence genes were predicted by applying Kleborate. Phenotypic and whole genome analyses revealed a high similarity of the study isolate 18-0005 to the recently reported antibiotic-susceptible hvKp isolate SB5881 from France and the "ancestral" strain Kp52.145; both were assigned to the ST66-K2 lineage. Comparative genomic analysis of the three plasmids showed that the 18-0005 plasmid II differs from SB5881 plasmid II by an additional 3 kb integrated fragment of plasmid I. Our findings demonstrate the genetic flexibility of hvKp and the occurrence of a strain of the clonal group CG66-K2 in Germany. Hence, it emphasizes the need to improve clinical awareness and infection monitoring of hvKp.
高毒力肺炎克雷伯菌（hvKp）是一种新型致病型，在欧洲鲜有报道。本研究对一株引起社区获得性感染的hvKp分离株进行了特征分析。2017年从一名患有扁桃体咽炎的德国患者身上获得了高黏液性菌株18 - 0005。进行了抗生素敏感性测试，并通过Illumina和Nanopore技术对基因组进行了测序。通过核心基因组多位点序列分型（cgMLST）和单核苷酸多态性（SNP）分析对全基因组数据进行了分析。应用Kleborate预测毒力基因。表型和全基因组分析显示，研究分离株18 - 0005与最近报道的来自法国的抗生素敏感型hvKp分离株SB5881以及“祖先”菌株Kp52.145高度相似；二者均属于ST66 - K2谱系。对三个质粒的比较基因组分析表明，18 - 0005质粒II与SB5881质粒II的差异在于其额外整合了一段3 kb的质粒I片段。我们的研究结果证明了hvKp的遗传灵活性以及德国出现了克隆群CG66 - K2的一个菌株。因此，它强调了提高对hvKp的临床认识和感染监测的必要性。
