Biology Department, College of Science, Jouf University, Sakaka, Saudi Arabia.
Department of Weed Science and Botany, University of Agriculture Peshawar, Peshawar, Pakistan.
PeerJ. 2022 Aug 2;10:e13623. doi: 10.7717/peerj.13623. eCollection 2022.
The influence of dry leachates of was tested on the seedling growth, photosynthesis, biochemical attributes, and gene expression of the economically important crops, including wheat ( L.), radish ( L.), barley ( L.) and arugula ( L.). Different concentrations (5%, 10%, 15%, 20%, and 25%) of stem extract (SE) and leaf extract (LE) of were prepared, and seedlings were allowed to grow in Petri plates for 8 days. The results showed that all plant species exhibited reduced germination rate, plant height, and fresh and dry weight due to leachates extracts of . Moreover, the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), exhibited differential regulation due to the extract treatment. The SOD was increased with increasing the concentration of extracts, while CAT and APX activities were decreased with increasing the extract concentrations. In addition, leachate extract treatment decrease chlorophyll content, photosynthesis, PSII activity, and water use efficiency, with evident effects at their higher concentrations. Furthermore, the content of proline, sugars, protein, total phenols, and flavonoids were reduced considerably due to leachates extract treatments. Furthermore, seedlings treated with high concentrations of LE increased the expression of genes. The present results lead to the conclusion that contains significant allelochemicals that interfere with the growth and development of the tested crop species and reduced the crops biomass and negatively affected other related parameters. However, further studies are suggested to determine the isolation and purification of the active compounds present in extracts.
对 产生的干淋滤液进行了测试，以观察其对包括小麦（ L.）、萝卜（ L.）、大麦（ L.）和芝麻菜（ L.）在内的经济重要作物的幼苗生长、光合作用、生化特性和基因表达的影响。不同浓度（5%、10%、15%、20%和 25%）的茎提取物（SE）和叶提取物（LE）制备后，让幼苗在培养皿中生长 8 天。结果表明，由于 的浸出物提取物，所有植物物种的发芽率、株高、鲜重和干重均降低。此外，由于提取物处理，抗氧化酶（如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX））的活性表现出不同的调节。随着提取物浓度的增加，SOD 增加，而 CAT 和 APX 活性随着提取物浓度的增加而降低。此外，浸出物提取物处理降低了叶绿素含量、光合作用、PSII 活性和水分利用效率，在较高浓度下效果更为明显。此外，由于浸出物提取物处理，脯氨酸、糖、蛋白质、总酚和类黄酮的含量明显降低。此外，用高浓度 LE 处理的幼苗增加了基因的表达。本研究结果表明， 含有显著的化感物质，这些物质会干扰受测作物物种的生长和发育，并降低作物生物量，对其他相关参数产生负面影响。然而，建议进一步研究以确定 提取物中存在的活性化合物的分离和纯化。
