Wohlgemuth Roland, Littlechild Jennifer
Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Lodz, Poland.
Swiss Coordination Committee for Biotechnology, Zurich, Switzerland.
Front Bioeng Biotechnol. 2022 Jul 22;10:958606. doi: 10.3389/fbioe.2022.958606. eCollection 2022.
The biosynthesis of metabolites from available starting materials is becoming an ever important area due to the increasing demands within the life science research area. Access to metabolites is making essential contributions to analytical, diagnostic, therapeutic and different industrial applications. These molecules can be synthesized by the enzymes of biological systems under sustainable process conditions. The facile synthetic access to the metabolite and metabolite-like molecular space is of fundamental importance. The increasing knowledge within molecular biology, enzyme discovery and production together with their biochemical and structural properties offers excellent opportunities for using modular cell-free biocatalytic systems. This reduces the complexity of synthesizing metabolites using biological whole-cell approaches or by classical chemical synthesis. A systems biocatalysis approach can provide a wealth of optimized enzymes for the biosynthesis of already identified and new metabolite molecules.
由于生命科学研究领域的需求不断增加，利用可用起始原料生物合成代谢物正成为一个日益重要的领域。获取代谢物对分析、诊断、治疗及不同的工业应用都做出了重要贡献。这些分子可在可持续的工艺条件下由生物系统的酶合成。便捷地合成代谢物及类似代谢物的分子空间至关重要。分子生物学、酶的发现与生产以及它们的生化和结构特性方面日益增长的知识，为使用模块化无细胞生物催化系统提供了绝佳机会。这降低了使用生物全细胞方法或经典化学合成方法合成代谢物的复杂性。系统生物催化方法可为已鉴定的和新的代谢物分子的生物合成提供大量优化的酶。
