Ramli Rosmaliza, Mohd Nafi Siti Norasikin, Ahmad Tarmidzi Nor Azura, Hasbullah Nurulezah, Ghani Nurhafizah
Oral Biology and Basic Sciences Unit, School of Dental Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia.
Department of Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia.
Saudi Dent J. 2022 Mar;34(3):155-166. doi: 10.1016/j.sdentj.2022.02.004. Epub 2022 Feb 26.
Despite advances in pain detection, diagnosis, and management, the prevalence of dental pain is still on the rise. Although dental pain is not directly related to fatal outcomes, the two most common types of dental pain-dental caries and dentin hypersensitivity-have a significant impact on an individual's quality of life. Understanding the mechanism of the pain pathway is one of the crucial steps in providing better treatment for these patients. Ion channels are critical biomolecules that have been the subject of dental study owing to their roles in the transmission and transduction of external stimuli, as well as in the control and perception of pain. Numerous immunohistochemical (IHC) staining approaches have also been used to identify the many ion channels implicated in peripheral pain signaling in dental pulp.
This review highlights the critical steps in IHC and its role in the detection of ion channels involved in the dental pain signaling pathway.
The key ion channels identified using IHC and whose functions have been widely researched in dental tissues are addressed in this review article.
尽管在疼痛检测、诊断和管理方面取得了进展，但牙疼痛的患病率仍在上升。虽然牙疼痛与致命后果没有直接关系，但两种最常见的牙疼痛类型——龋齿和牙本质过敏——对个人生活质量有重大影响。了解疼痛通路的机制是为这些患者提供更好治疗的关键步骤之一。离子通道是关键的生物分子，由于它们在外部刺激的传递和转导以及疼痛的控制和感知中的作用，一直是牙科研究的主题。许多免疫组织化学（IHC）染色方法也被用于识别牙髓外周疼痛信号中涉及的许多离子通道。
本综述强调了免疫组织化学中的关键步骤及其在检测参与牙疼痛信号通路的离子通道中的作用。
本文综述了使用免疫组织化学鉴定的关键离子通道，其功能已在牙组织中得到广泛研究。
