Sánchez-Herrero Estela, Serna-Blasco Roberto, Robado de Lope Lucia, González-Rumayor Víctor, Romero Atocha, Provencio Mariano
Liquid Biopsy Laboratory. Biomedical Sciences Research Institute Puerta de Hierro-Majadahonda, Majadahonda, Spain.
+D Department, Atrys Health, Barcelona, Spain.
Front Oncol. 2022 Jul 20;12:943253. doi: 10.3389/fonc.2022.943253. eCollection 2022.
Cancer cells release nucleic acids, freely or associated with other structures such as vesicles into body fluids, including blood. Among these nucleic acids, circulating tumor DNA (ctDNA) has emerged as a minimally invasive biomarker for tumor molecular profiling. However, certain biological characteristics of ctDNA are still unknown. Here, we provide an overview of the current knowledge about ctDNA biological features, including size and structure as well as the mechanisms of ctDNA shedding and clearance, and the physio-pathological factors that determine ctDNA levels. A better understanding of ctDNA biology is essential for the development of new methods that enable the analysis of ctDNA.
癌细胞会将核酸释放到包括血液在内的体液中，这些核酸可以是游离状态，也可以与囊泡等其他结构结合。在这些核酸中，循环肿瘤DNA（ctDNA）已成为一种用于肿瘤分子分析的微创生物标志物。然而，ctDNA的某些生物学特性仍不清楚。在此，我们概述了目前关于ctDNA生物学特征的知识，包括其大小和结构、ctDNA释放与清除的机制以及决定ctDNA水平的生理病理因素。更好地理解ctDNA生物学对于开发能够分析ctDNA的新方法至关重要。
