Wang Jian, Liu Xiao-Fen, Zhang Hui-Qin, Allan Andrew C, Wang Wen-Qiu, Yin Xue-Ren
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou Zhejiang, 310058, China.
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou Zhejiang, 310021, China.
Hortic Res. 2022 May 17;9:uhac116. doi: 10.1093/hr/uhac116. eCollection 2022.
Levels of ethylene, implicated in the induction of fruit ripening in a diverse array of plants, are influenced by genetic and environmental factors, such as other plant hormones. Among these, salicylic acid (SA) and its derivative, acetylsalicylic acid (ASA), have been demonstrated to inhibit ethylene biosynthesis in fruit, yet the underlying regulatory mechanisms remain elusive. Here, we showed that treatment with exogenous ASA dramatically reduced ethylene production, as well as activities of ACC synthase (ACS) and ACC oxidase (ACO), in kiwifruit tissues. Comparative transcriptome analysis indicated the differential expression of ethylene biosynthetic genes ( and ). A screen of transcription factors indicated that AdERF105L and AdWRKY29 were ASA-responsive regulators of and , respectively In addition to these genes, and were abundantly expressed in both ASA-treated and control tissues. AdACS3 protein was phosphorylated and stabilized by AdMPK16, a mitogen-activated protein kinase, while AdACO3 activity was enhanced by AdAP, an aspartic peptidase. Exogenous ASA downregulated and , thereby influencing ethylene biosynthesis at a post-transcriptional level. These findings led us to propose a multidimensional system for inhibition of ethylene biosynthesis by ASA, inducing differential expression of some ethylene biosynthesis genes, as well as differential effects on protein activity on other targets.
乙烯水平在多种植物果实成熟诱导过程中发挥作用，它受遗传和环境因素影响，比如其他植物激素。其中，水杨酸（SA）及其衍生物乙酰水杨酸（ASA）已被证明可抑制果实中的乙烯生物合成，但其潜在调控机制仍不清楚。在此，我们表明用外源ASA处理可显著降低猕猴桃组织中的乙烯产量以及ACC合成酶（ACS）和ACC氧化酶（ACO）的活性。比较转录组分析表明乙烯生物合成基因（ 和 ）的差异表达。转录因子筛选表明AdERF105L和AdWRKY29分别是 和 的ASA响应调节因子。除了这些基因， 和 在ASA处理和对照组织中均大量表达。AdACS3蛋白被丝裂原活化蛋白激酶AdMPK16磷酸化并稳定，而AdACO3活性被天冬氨酸肽酶AdAP增强。外源ASA下调 和 ，从而在转录后水平影响乙烯生物合成。这些发现使我们提出一个多维系统，用于ASA抑制乙烯生物合成，诱导一些乙烯生物合成基因的差异表达，以及对其他靶点的蛋白质活性产生不同影响。
