Ali Barkat, Kumar Sujon, Sui Xiyu, Niu Jianpo, Yang Junqi, Zheng Mengni, Tang Yi, Li Huanxiu
College of Horticulture, Sichuan Agricultural University, Chengdu, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
Front Plant Sci. 2025 Jun 13;16:1589706. doi: 10.3389/fpls.2025.1589706. eCollection 2025.
Cold stress severely limits the growth and productivity of common bean ( L.) seedlings, particularly during early development. Exogenous application of acetylsalicylic acid (ASA) has proven to be an effective strategy for enhancing cold tolerance. This study investigates the usefulness of exogenous ASA in enhancing cold tolerance in common bean seedlings exposed to cold stress of 5°C for 12 and 24 hours, along with a control (0h). ASA treatments (1 mM and 2 mM) significantly improved critical physiological and biochemical parameters, including photosynthesis, chlorophyll and carotenoid concentrations, oxidative stress markers, malondialdehyde, electrical conductivity, total soluble proteins (MDA, EC, SP), and antioxidant enzyme activity. Under cold stress, ASA2 constantly outperformed the other treatments. Following a 12-hour period, ASA2 showed increased chlorophyll concentrations (8.88%) and augmented levels (21.25%), alongside reducing MDA by 24.96% and SP by 67.1%. After 24 h, ASA2 demonstrated a slight increase in chlorophyll (4.26%) and raised (25.33%), with a significant reduction in MDA (16.5%) and SP (68.3%). ASA1 showed enhancements, mainly in (39.89% at 12 h) and antioxidant enzymes, with notable increases in SOD (113.17% at 12 h) and POD (110.98% at 12 h). Correlation studies indicated significant positive relationships between antioxidant enzyme activity such as, superoxide dismutase, catalase, peroxidase, ascorbate peroxidase (SOD, CAT, POD, and APX) and photosynthetic efficiency. Principal component analysis (PCA) identified ASA2 as the most effective treatment for enhancing stress resilience, accounting for the largest variance in membrane integrity and reduction of oxidative stress. Network analysis further confirmed that ASA2 strengthened the connections between photosynthesis and antioxidant activity, with more resilient and interconnected nodes indicating improved stress adaptability. At 2 mM, ASA upregulated antioxidant genes () and photosynthesis genes (), reducing cold-induced oxidative stress and preserving chloroplast function, thereby enhancing cold tolerance and crop resilience under climate stress.
低温胁迫严重限制了普通菜豆（Phaseolus vulgaris L.）幼苗的生长和生产力，尤其是在早期发育阶段。外源施用乙酰水杨酸（ASA）已被证明是增强耐寒性的有效策略。本研究调查了外源ASA对暴露于5°C低温胁迫12小时和24小时的普通菜豆幼苗以及对照（0小时）增强耐寒性的作用。ASA处理（1 mM和2 mM）显著改善了关键的生理和生化参数，包括光合作用、叶绿素和类胡萝卜素浓度、氧化应激标志物、丙二醛、电导率、总可溶性蛋白（MDA、EC、SP）以及抗氧化酶活性。在低温胁迫下，ASA2的表现始终优于其他处理。处理12小时后，ASA2的叶绿素浓度增加了8.88%，光合效率提高了21.25%，同时MDA含量降低了24.96%，SP含量降低了67.1%。处理24小时后，ASA2的叶绿素略有增加（4.26%），光合效率提高了25.33%（原文此处“raised ”后面似乎缺少内容），MDA显著降低（16.5%），SP降低（68.3%）。ASA1也有增强作用，主要体现在光合效率（12小时时提高39.89%）和抗氧化酶方面，SOD（12小时时增加113.17%）和POD（12小时时增加110.98%）显著增加。相关性研究表明，超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶（SOD、CAT、POD和APX）等抗氧化酶活性与光合效率之间存在显著正相关。主成分分析（PCA）确定ASA2是增强胁迫恢复力最有效的处理，在膜完整性和氧化应激降低方面解释了最大的方差。网络分析进一步证实，ASA2加强了光合作用和抗氧化活性之间的联系，更具弹性和相互连接的节点表明胁迫适应性提高。在2 mM时，ASA上调了抗氧化基因（原文此处“()”内似乎缺少基因名称）和光合作用基因（原文此处“()”内似乎缺少基因名称），减少了低温诱导的氧化应激并维持叶绿体功能，从而提高了低温耐受性和作物在气候胁迫下的恢复力。
