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盘状溶素的双重功能，一种由Nm-76菌株产生的具有N端盘状结构域的胆固醇依赖性细胞溶素。

Dual functions of discoidinolysin, a cholesterol-dependent cytolysin with N-terminal discoidin domain produced from strain Nm-76.

作者信息

Tabata Atsushi, Matsumoto Airi, Fujimoto Ai, Ohkura Kazuto, Ikeda Takuya, Oda Hiroki, Yokohata Shuto, Kobayashi Miho, Tomoyasu Toshifumi, Takao Ayuko, Ohkuni Hisashi, Nagamune Hideaki

机构信息

Department of Bioengineering, Division of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University Graduate School, Tokushima, Japan.

Department of Biological Science and Technology, Life System, Institute of Technology and Science, Tokushima University Graduate School, Tokushima, Japan.

出版信息

J Oral Microbiol. 2022 Aug 1;14(1):2105013. doi: 10.1080/20002297.2022.2105013. eCollection 2022.

DOI:10.1080/20002297.2022.2105013
PMID:35937899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9351568/
Abstract

BACKGROUND

Some strains of exhibit β-hemolysis due to the β-hemolytic activity of cholesterol-dependent cytolysin (CDC). Recently, a gene encoding an atypical lectinolysin-related CDC was found in strain Nm-76. However, the product of this gene remains uncharacterized. We aimed to characterize this atypical CDC and its molecular functions and contribution to the pathogenicity of strain Nm-76.

METHODS

Phylogenetic analysis of the CDC gene was conducted based on the web-deposited information. The molecular characteristics of CDC were investigated using a gene-deletion mutant strain and recombinant proteins expressed in .

RESULTS

The gene encoding CDC found in Nm-76 and its homolog are distributed among many strains. This CDC is phylogenetically different from other previously characterized CDCs, such as -derived human platelet aggregation factor (Sm-hPAF)/lectinolysin and mitilysin. Because this CDC possesses an additional N-terminal domain, including a discoidin motif, it was termed discoidinolysin (DLY). In addition to the preferential lysis of human cells, DLY displayed N-terminal domain-dependent facilitation of human erythrocyte aggregation and intercellular associations between human cells.

CONCLUSION

DLY functions as a hemolysin/cytolysin and erythrocyte aggregation/intercellular association molecule. This dual-function DLY could be an additional virulence factor in .

摘要

背景

某些菌株由于胆固醇依赖性细胞溶素（CDC）的β-溶血活性而表现出β-溶血。最近，在菌株Nm-76中发现了一个编码非典型凝集素溶素相关CDC的基因。然而，该基因的产物仍未得到表征。我们旨在表征这种非典型CDC及其分子功能，以及它对菌株Nm-76致病性的贡献。

方法

基于网络存储信息对CDC基因进行系统发育分析。使用基因缺失突变株和在大肠杆菌中表达的重组蛋白研究CDC的分子特征。

结果

在Nm-76中发现的编码CDC的基因及其同源物分布在许多菌株中。这种CDC在系统发育上与其他先前表征的CDC不同，例如链球菌来源的人血小板聚集因子（Sm-hPAF）/凝集素溶素和米氏溶素。由于这种CDC具有一个额外的N端结构域，包括一个盘状结构域基序，因此被称为盘状溶素（DLY）。除了对人细胞的优先裂解外，DLY还表现出N端结构域依赖性促进人红细胞聚集和人细胞间的细胞间结合。

结论

DLY作为溶血素/细胞溶素以及红细胞聚集/细胞间结合分子发挥作用。这种具有双重功能的DLY可能是肺炎链球菌中的另一种毒力因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/e5270f972339/ZJOM_A_2105013_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/f6fa81d1e5ab/ZJOM_A_2105013_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/198c8e3b9c4a/ZJOM_A_2105013_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/f934949a420c/ZJOM_A_2105013_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/82c1a8587e4a/ZJOM_A_2105013_F0004_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/bfb2a6580fd5/ZJOM_A_2105013_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/5c65a76b5516/ZJOM_A_2105013_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/3f320052985e/ZJOM_A_2105013_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/e5270f972339/ZJOM_A_2105013_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/f6fa81d1e5ab/ZJOM_A_2105013_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/198c8e3b9c4a/ZJOM_A_2105013_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/f934949a420c/ZJOM_A_2105013_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/82c1a8587e4a/ZJOM_A_2105013_F0004_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/bfb2a6580fd5/ZJOM_A_2105013_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/5c65a76b5516/ZJOM_A_2105013_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/3f320052985e/ZJOM_A_2105013_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39fe/9351568/e5270f972339/ZJOM_A_2105013_F0008_OC.jpg

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