Ullah Md Asad, Islam Nafisa Nawal, Moin Abu Tayab, Park Su Hyun, Kim Bonglee
Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh.
Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chattogram, Bangladesh.
Front Genet. 2022 Jul 22;13:935286. doi: 10.3389/fgene.2022.935286. eCollection 2022.
This study explored the prognostic and therapeutic potentials of multiple Proteasome 26S Subunit, ATPase () family of genes () in lung adenocarcinoma (LUAD) diagnosis and treatment. All the s were found to be differentially expressed (upregulated) at the mRNA and protein levels in LUAD tissues. The promoter and multiple coding regions of s were reported to be differentially and distinctly methylated, which may serve in the methylation-sensitive diagnosis of LUAD patients. Multiple somatic mutations (alteration frequency: 0.6-2%) were observed along the coding regions in LUAD tissues that could assist in the high-throughput screening of LUAD patients. A significant association between the overexpression and LUAD patients' poor overall and relapse-free survival ( < 0.05; HR: >1.3) and individual cancer stages ( < 0.001) was discovered, which justifies s as the ideal targets for LUAD diagnosis. Multiple immune cells and modulators (i.e., CD274 and IDO1) were found to be associated with the expression levels of in LUAD tissues that could aid in formulating -based diagnostic measures and therapeutic interventions for LUAD. Functional enrichment analysis of neighbor genes of s in LUAD tissues revealed different genes (i.e., and ) previously known to be involved in oncogenic processes and metastasis are co-expressed with s, which could also be investigated further. Overall, this study recommends that and their transcriptional and translational products are potential candidates for LUAD diagnostic and therapeutic measure discovery.
本研究探讨了多种蛋白酶体26S亚基ATP酶（）家族基因（）在肺腺癌（LUAD）诊断和治疗中的预后及治疗潜力。研究发现，所有这些基因在LUAD组织的mRNA和蛋白质水平均呈差异表达（上调）。据报道，这些基因的启动子和多个编码区域存在差异且明显的甲基化，这可能有助于LUAD患者的甲基化敏感性诊断。在LUAD组织的编码区域观察到多个体细胞突变（改变频率：0.6 - 2%），这有助于对LUAD患者进行高通量筛查。研究发现这些基因的过表达与LUAD患者较差的总生存期和无复发生存期（<0.05；HR：>1.3）以及个体癌症分期（<0.001）之间存在显著关联，这证明这些基因是LUAD诊断的理想靶点。在LUAD组织中发现多种免疫细胞和调节因子（即CD274和IDO1）与这些基因的表达水平相关，这有助于制定基于这些基因的LUAD诊断措施和治疗干预方案。对LUAD组织中这些基因的邻近基因进行功能富集分析发现，先前已知参与致癌过程和转移的不同基因（即和）与这些基因共表达，这也可进一步研究。总体而言，本研究表明这些基因及其转录和翻译产物是发现LUAD诊断和治疗措施的潜在候选对象。
