Sun Bo, Wang Jun, Zhu Yan-Feng, Li Zhen-Yang, Xiang Jian-Bin, Chen Zong-You, He Zhi-Gang, Gu Xiao-Dong
Department of Gastric Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Arch Med Sci. 2020 Apr 15;19(3):626-632. doi: 10.5114/aoms.2020.94487. eCollection 2023.
Translocase of the inner mitochondrial membrane 50 (TIMM50) is universally considered to play a key role in several malignancies. However, its role in predicting colorectal cancer (CRC) patient prognosis remains unclear.
A total of 192 CRC patients (123 men and 69 women) who underwent radical resection participated in this study. The patients were followed up every 3 months after surgery for 5 years. TIMM50 expression in tumour tissues was measured by quantitative real-time PCR, Western blotting and immunohistochemistry. TIMM50 expression was studied to assess correlations with clinicopathological factors and survival time.
TIMM50 expression increased significantly in CRC tumour tissues. Moreover, high TIMM50 expression was related to pathologic stage ( = 0.043), N stage ( = 0.048) and distant metastasis ( = 0.015), but TIMM50 expression was not related to other clinical factors. A Kaplan-Meier survival analysis indicated that patients with low TIMM50 expression had a longer overall survival than those with high TIMM50 expression ( = 0.002). Furthermore, distant metastasis and high TIMM50 expression were confirmed as independent prognostic factors for the overall survival of CRC patients in a multivariate analysis ( = 0.003).
TIMM50 may be a key factor for monitoring CRC and a new prognosis indicator for CRC patients.
线粒体内膜转位酶50（TIMM50）普遍被认为在多种恶性肿瘤中起关键作用。然而，其在预测结直肠癌（CRC）患者预后方面的作用仍不明确。
共有192例行根治性切除术的CRC患者（123例男性和69例女性）参与本研究。术后每3个月对患者进行随访，持续5年。通过定量实时PCR、蛋白质免疫印迹法和免疫组织化学法检测肿瘤组织中TIMM50的表达。研究TIMM50表达以评估其与临床病理因素及生存时间的相关性。
CRC肿瘤组织中TIMM50表达显著增加。此外，高TIMM50表达与病理分期（P = 0.043）、N分期（P = 0.048）和远处转移（P = 0.015）相关，但TIMM50表达与其他临床因素无关。Kaplan-Meier生存分析表明，TIMM50低表达患者的总生存期长于TIMM50高表达患者（P = 0.002）。此外，多因素分析证实远处转移和高TIMM50表达是CRC患者总生存的独立预后因素（P = 0.003）。
TIMM50可能是监测CRC的关键因素及CRC患者的新预后指标。
