Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.
Professor, Department of Periodontology, School of Dentistry, Universidad Cientifica del Sur, Lima, Peru.
Biofouling. 2022 Jul;38(6):656-662. doi: 10.1080/08927014.2022.2070431. Epub 2022 Aug 8.
Anodization is a routine industrial galvanic method that produces a titanium oxide layer on the surface of titanium. Considering the possibility that this technique could influence microbial adsorption and colonization, this study was conducted to evaluate the impact of a process of anodization applied to a titanium surface on the microbial profile of multispecies subgingival biofilm. Titanium discs produced by using two different processes-conventional and Anodization-were divided into two groups: conventional titanium discs with machined surface (cpTi) Control Group and titanium discs with anodic oxidation treatment (anTi) Test Group. Subgingival biofilm composed of 33 species was formed on the titanium discs that were positioned vertically in 96-well plates, for 7 days. The proportions and the counts of microbial species were determined using a DNA-DNA hybridization technique, and data were evaluated using Mann-Whitney test ( < 0.05). Mean total bacterial counts were lower in Test Group in comparison with Control Group ( < 0.05). Nine bacterial species differed significantly, and were found in higher levels in Control Group in comparison with Test Group, including , In conclusion, titanium discs with anodization could alter the microbial profile of the biofilm formed around them. Further clinical studies should be conducted to confirm the clinical impact of these findings.
阳极氧化是一种常规的工业电镀方法，可在钛表面生成一层氧化钛。考虑到该技术可能影响微生物的吸附和定植，本研究旨在评估应用于钛表面的阳极氧化工艺对多物种龈下生物膜微生物谱的影响。使用两种不同工艺（常规和阳极氧化）生产的钛片分为两组：经机械加工表面处理的常规钛片（cpTi）对照组和经阳极氧化处理的钛片（anTi）实验组。将由 33 个物种组成的龈下生物膜垂直置于 96 孔板中的钛片上，培养 7 天。采用 DNA-DNA 杂交技术确定微生物种类的比例和计数，并使用曼-惠特尼检验（ < 0.05）进行评估。实验组的总细菌计数明显低于对照组（ < 0.05）。有 9 种细菌的差异具有统计学意义，且在对照组中的水平明显高于实验组，包括 ， 。结论：阳极氧化钛片可能会改变其周围生物膜的微生物谱。应进行进一步的临床研究以证实这些发现的临床意义。
