Tanaka Caio Junji, Rodrigues José Augusto, Pingueiro João Marcos Spessoto, Macedo Tatiane Tiemi, Feres Magda, Shibli Jamil Awad, Bueno-Silva Bruno
School of Dentistry, Mogi das Cruzes University, Mogi das Cruzes 08780-911, Sao Paulo, Brazil.
Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS/UNG, Guarulhos 07011-010, Sao Paulo, Brazil.
Pharmaceutics. 2023 Jun 14;15(6):1727. doi: 10.3390/pharmaceutics15061727.
Bioactive materials were developed with the ability to release fluoride and provide some antimicrobial potential, to be widely used in dentistry today. However, few scientific studies have evaluated the antimicrobial activity of bioactive surface pre-reacted glass (S-PRG) coatings (PRG Barrier Coat, Shofu, Kyoto, Japan) on periodontopathogenic biofilms. This study evaluated the antibacterial activity of S-PRG fillers on the microbial profile of multispecies subgingival biofilms. A Calgary Biofilm Device (CBD) was used to grow a 33-species biofilm related to periodontitis for 7 days. The S-PRG coating was applied on CBD pins from the test group and photo-activated (PRG Barrier Coat, Shofu), while the control group received no coating. Seven days after treatment, the total bacterial counts, metabolic activity, and microbial profile of the biofilms were observed using a colorimetric assay and DNA-DNA hybridization. Statistical analyses were applied; namely, the Mann-Whitney, Kruskal-Wallis, and Dunn's post hoc tests. The bacterial activity of the test group was reduced by 25.7% compared with that of the control group. A statistically significant reduction was observed for the counts of 15 species: , , , , , , , , , , , , , , and ( ≤ 0.05). The bioactive coating containing S-PRG modified the composition of the subgingival biofilm in vitro, thereby decreasing colonization by pathogens.
生物活性材料已被开发出来，具有释放氟化物的能力并具备一定的抗菌潜力，如今在牙科领域得到广泛应用。然而，很少有科学研究评估生物活性表面预反应玻璃（S-PRG）涂层（PRG Barrier Coat，松风株式会社，日本京都）对牙周致病生物膜的抗菌活性。本研究评估了S-PRG填料对多物种龈下生物膜微生物谱的抗菌活性。使用卡尔加里生物膜装置（CBD）培养与牙周炎相关的33物种生物膜7天。将S-PRG涂层应用于试验组的CBD针上并进行光活化（PRG Barrier Coat，松风株式会社），而对照组未进行涂层处理。处理7天后，使用比色法和DNA-DNA杂交观察生物膜的总细菌计数、代谢活性和微生物谱。进行了统计分析，即曼-惠特尼检验、克鲁斯卡尔-沃利斯检验和邓恩事后检验。试验组的细菌活性与对照组相比降低了25.7%。观察到15个物种的计数有统计学显著降低： ， ， ， ， ， ， ， ， ， ， ， ， ， 以及 （ ≤ 0.05）。含有S-PRG的生物活性涂层在体外改变了龈下生物膜的组成，从而减少了病原体的定植。
