Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang 830046, China.
Food Funct. 2022 Aug 30;13(17):8967-8976. doi: 10.1039/d2fo00753c.
Chickpeas are an important source of flavonoids in the human diet, and researchers have demonstrated that flavonoids have antidiabetic compositions in chickpeas. Because the NAD/NADH redox balance is heavily perturbed in diabetes and complex I is the only site for NADH oxidation and NAD regeneration, in the present study, mitochondrial complex I was used as a target for anti-diabetes. The objective of this study was to investigate the effects of a crude chickpea flavonoid extract (CCFE) on NAD/NADH redox imbalance and mitochondrial complex I dysfunction in the pancreas as well as oxidative stress in type 2 diabetes mellitus (T2DM) rats. Our results demonstrated that the degree of NAD/NADH redox imbalance in the pancreas of T2DM rats was alleviated by CCFE, which is likely attributed to the inhibition of the polyol pathway and the decrease in poly ADP ribose polymerase (PARP) and sirtuin 3 (Sirt3) activities. Moreover, mitochondrial complex I dysfunction in the pancreas of T2DM rats was ameliorated by CCFE through the suppression of the activity of complex I. Furthermore, CCFE treatment could attenuate oxidative stress in T2DM rats, which was proven by the reduction in hydrogen peroxide (HO) and malondialdehyde (MDA) as well as the upregulation of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in serum. CCFE treatment significantly improved dyslipidemia in T2DM rats.
鹰嘴豆是人类饮食中黄酮类化合物的重要来源，研究人员已经证明，黄酮类化合物在鹰嘴豆中具有抗糖尿病的成分。由于 NAD/NADH 氧化还原平衡在糖尿病中受到严重干扰，并且复合体 I 是 NADH 氧化和 NAD 再生的唯一部位，因此在本研究中，线粒体复合体 I 被用作抗糖尿病的靶点。本研究的目的是研究粗鹰嘴豆类黄酮提取物（CCFE）对 2 型糖尿病（T2DM）大鼠胰腺中 NAD/NADH 氧化还原失衡和线粒体复合体 I 功能障碍以及氧化应激的影响。我们的结果表明，CCFE 缓解了 T2DM 大鼠胰腺中 NAD/NADH 氧化还原失衡的程度，这可能归因于多元醇途径的抑制以及多聚 ADP 核糖聚合酶（PARP）和 Sirtuin 3（Sirt3）活性的降低。此外，CCFE 通过抑制复合体 I 的活性改善了 T2DM 大鼠胰腺中的线粒体复合体 I 功能障碍。此外，CCFE 处理可减轻 T2DM 大鼠的氧化应激，这可通过减少血清中的过氧化氢（HO）和丙二醛（MDA）以及上调谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）来证明。CCFE 处理显著改善了 T2DM 大鼠的血脂异常。
