Zhou Keqiang, Xiao Shiqi, Cao Shenyi, Zhao Caiyun, Zhang Minwei, Fu Yinghua
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang 830046, China.
Food Chem X. 2024 Jul 6;23:101630. doi: 10.1016/j.fochx.2024.101630. eCollection 2024 Oct 30.
Chickpea ( L) is a significant dietary source of flavonoids and the hypoglycemic activity were investigated in this study. Firstly, total twenty nine chickpea flavonoids were identified by UPLC-MS/MS with ononin, cyanidin-3-O-glucoside, astragalin, cynaroside, kaempferol-3-O-rutinoside, biochanin A, and daidzin being the most abundant among them. Our results demonstrated that chickpea flavonoids regulated glucose metabolism and lipid metabolism, and reduced oxidative stress in insulin resistance HepG2 cells. Furthermore, insulin resistance was ameliorated by chickpea flavonoids through the activation of insulin receptor substrate1 (IRS1), phosphoinositide 3-kinase (PI3K), and phosphorylated protein kinase B (Akt) in HepG2 cells. More importantly, key differential metabolites include L-tryptophan, L-tyrosine, l-glutamine and linoleic acid were reserved by chickpea flavonoids and correlated with glucolipid metabolism and oxidative stress in IR-HepG2 cells. In conclusion, these results indicated that chickpea flavonoids might act as potential natural products regulating insulin resistance in HepG2 cells.
鹰嘴豆（L）是黄酮类化合物的重要膳食来源，本研究对其降血糖活性进行了调查。首先，通过超高效液相色谱-串联质谱法（UPLC-MS/MS）鉴定出总共29种鹰嘴豆黄酮类化合物，其中染料木苷、花青素-3-O-葡萄糖苷、紫云英苷、刺槐苷、山奈酚-3-O-芸香糖苷、鹰嘴豆芽素A和大豆苷最为丰富。我们的结果表明，鹰嘴豆黄酮类化合物调节葡萄糖代谢和脂质代谢，并降低胰岛素抵抗HepG2细胞中的氧化应激。此外，鹰嘴豆黄酮类化合物通过激活HepG2细胞中的胰岛素受体底物1（IRS1）、磷脂酰肌醇3-激酶（PI3K）和磷酸化蛋白激酶B（Akt）来改善胰岛素抵抗。更重要的是，鹰嘴豆黄酮类化合物保留了关键差异代谢物，包括L-色氨酸、L-酪氨酸、L-谷氨酰胺和亚油酸，这些代谢物与IR-HepG2细胞中的糖脂代谢和氧化应激相关。总之，这些结果表明，鹰嘴豆黄酮类化合物可能作为调节HepG2细胞胰岛素抵抗的潜在天然产物。
