Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
Centre for Research in Nanotechnology & Science (CRNTS), Indian Institute of Technology Bombay, Powai, Mumbai, India.
Proteomics Clin Appl. 2022 Nov;16(6):e2100111. doi: 10.1002/prca.202100111. Epub 2022 Sep 18.
To identify the specific diagnostic biomarkers related to pituitary adenomas (PAs), we performed serological antibody profiles for three types of PAs, namely Acromegaly, Cushing's and Nonfunctional Pituitary Adenomas (NFPAs), using the human proteome (HuProt) microarray. This is the first study describing the serum autoantibody profile of PAs.
We performed serological autoantibody profiling of four healthy controls, four Acromegaly, three Cushing's and three NFPAs patient samples to obtain their autoantibody profiles, which were used for studying expression, interaction and altered biological pathways. Further, significant autoantibodies of PAs were compared with data available for glioma, meningioma and AAgAtlas for their specificity.
Autoantibody profile of PAs led to the identification of differentially expressed significant proteins such as AKNAD1 (AT-Hook Transcription Factor [AKNA] Domain Containing 1), NINJ1 (Nerve injury-induced protein 1), L3HYPDH (Trans-3-hydroxy-L-proline dehydratase), RHOG (Rho-related GTP-binding protein) and PTP4A1 (Protein Tyrosine Phosphatase Type IVA 1) in Acromegaly. Protein ABR (Active breakpoint cluster region-related protein), ST6GALNAC6 (ST6 N-acetylgalactosaminide alpha-2, 6-sialyltransferase 6), NOL3 (Nucleolar protein 3), ANXA8 (Annexin A8) and POLR2H (RNA polymerase II, I and III subunit H) showed an antigenic response in Cushing's patient's serum samples. Protein dipeptidyl peptidase 3 (DPP3) and reticulon-4 (RTN4) exhibited a very high antigenic response in NFPA patients. These proteins hold promise as potential autoantibody biomarkers in PAs.
为了鉴定与垂体腺瘤(PA)相关的特定诊断生物标志物,我们使用人类蛋白质组(HuProt)微阵列对三种类型的 PA(肢端肥大症、库欣病和无功能垂体腺瘤(NFPAs))进行了血清抗体谱分析。这是首次描述 PA 的血清自身抗体谱的研究。
我们对 4 名健康对照者、4 名肢端肥大症患者、3 名库欣病患者和 3 名 NFPAs 患者的样本进行了血清自身抗体谱分析,以获得他们的自身抗体谱,用于研究表达、相互作用和改变的生物学途径。此外,还将 PA 的显著自身抗体与胶质瘤、脑膜瘤和 AAgAtlas 的可用数据进行了比较,以评估其特异性。
PA 的自身抗体谱导致鉴定出差异表达的显著蛋白,如 AKNAD1(AKNA 结构域包含蛋白 1)、NINJ1(神经损伤诱导蛋白 1)、L3HYPDH(反-3-羟脯氨酸脱水酶)、RHOG(Rho 相关 GTP 结合蛋白)和 PTP4A1(蛋白酪氨酸磷酸酶 4A1)在肢端肥大症患者中。蛋白 ABR(活跃断点簇区域相关蛋白)、ST6GALNAC6(ST6 N-乙酰半乳糖胺 α-2,6-唾液酸转移酶 6)、NOL3(核仁蛋白 3)、ANXA8(膜联蛋白 A8)和 POLR2H(RNA 聚合酶 II、I 和 III 亚基 H)在库欣病患者的血清样本中表现出抗原反应。蛋白二肽基肽酶 3(DPP3)和 reticulon-4(RTN4)在 NFPA 患者中表现出非常高的抗原反应。这些蛋白有望成为 PA 的潜在自身抗体生物标志物。