冷藏胰岛的代谢

Metabolism of cold-stored pancreatic islets.

作者信息

Frankel B J, Gylfe E, Hellman B, Idahl L A, Landström U, Løvtrup S, Sehlin J

出版信息

Diabetologia. 1978 Sep;15(3):187-90. doi: 10.1007/BF00421237.

Abstract

A previous study showed that the ability of glucose to stimulate insulin release was retained in islets stored at 8 degrees C for one week provided that glucose was present in a high concentration in the storage medium. The metabolic properties of islets stored in the cold have now been further explored in an attempt to clarify the protective effect of glucose. During storage in the cold the islet formation of 3H2O from (5--3H) glucose and oxygen consumption were only a few per cent of that of fresh islets whereas the putake of 86Rb+ was 20--48%. Rewarming the cold-stored islets to 37 degrees C after one week of cold-storage restored the 86Rb+ uptake, the formation of 3H2O and 14CO2 from labelled glucose and oxygen consumption to 75, 80, 60 and 40% respectively of fresh islet levels. The results emphasize the usefulness of cold-storage for preservation of functionally intact isolated islets.

摘要

先前的一项研究表明，只要储存培养基中存在高浓度葡萄糖，在8摄氏度下储存一周的胰岛仍保留葡萄糖刺激胰岛素释放的能力。现在，为了阐明葡萄糖的保护作用，对冷藏胰岛的代谢特性进行了进一步研究。在冷藏期间，（5-³H）葡萄糖生成³H₂O的胰岛形成以及氧消耗仅为新鲜胰岛的百分之几，而⁸⁶Rb⁺摄取量为20%-48%。冷藏一周后将冷藏的胰岛复温至37摄氏度，⁸⁶Rb⁺摄取量、标记葡萄糖生成³H₂O和¹⁴CO₂的量以及氧消耗分别恢复到新鲜胰岛水平的75%、80%、60%和40%。结果强调了冷藏对于保存功能完整的分离胰岛的有用性。