Key Laboratory of Functional Molecules Analysis and Biotransformation of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, China.
The First People's Hospital of Yunnan Province, Kunming, 650034, China.
Phytochemistry. 2022 Oct;202:113359. doi: 10.1016/j.phytochem.2022.113359. Epub 2022 Aug 5.
Ten undescribed C polyketide phialocetones A-J, featuring twelve-, six- and five-membered lactone moieties, were isolated from a rhizospheric soil-derived Phialocephala sp. YUD18001 associated with Gastrodia elata. Their structures were established by NMR spectroscopic analysis and HRMS, while their absolute configurations were determined by computational methods and chemical reactions. All isolated compounds were evaluated for their anti-inflammatory and cytotoxic activities. As a result, phialocetone D exhibited moderate effects against NO production in lipopolysaccharide (LPS)-induced RAW264.7 cells with an IC value of 14.77 μM, while phialocetone E showed cytotoxicity against HL-60 and SW480 cell lines with IC values of 19.04 and 10.22 μM, respectively.
从与天麻共生的土壤来源的拟青霉属 YUD18001 中分离得到 10 个未被描述的 C 聚酮类 phialocetones A-J，其具有十二元、六元和五元内酯部分。通过 NMR 光谱分析和高分辨质谱（HRMS）确定了它们的结构，而它们的绝对构型则通过计算方法和化学反应确定。所有分离得到的化合物都进行了抗炎和细胞毒性活性评估。结果表明，phialocetone D 对脂多糖（LPS）诱导的 RAW264.7 细胞中 NO 产生具有中等抑制作用，IC 值为 14.77 μM，而 phialocetone E 对 HL-60 和 SW480 细胞系表现出细胞毒性，IC 值分别为 19.04 和 10.22 μM。
