LC-MS/MS metabolomics profiling of L. methanolic extract; antimicrobial and with wound healing studies on infected rat skin wound.

LC-MS/MS analysis of L. methanolic extract (GHME), revealed the identification of 25 metabolites. Ursolic acid , 2-hydroxyursolic acid or corosolic acid , 2-hydroxyursolic acid or epi-corosolic , luteolin 7---D-glucopyranoside and rosmarinic acid were isolated and identified using spectroscopy. Antibacterial activity of GHME against multi drug resistance clinical isolates was measured. Minimum inhibitory concentrations (MICs) were ranged from 62.5 to 500 µg/ml. wound healing potential of 2%, and 5% GHME prepared hydrogels were criticized on infected wound rat model. 5% GHME prepared hydrogel treated group showed significant ( < 0.05) shrinkage of their colony forming unit/ml (CFU/ml) values in comparison with standard Fucidin. Meanwhile, wound closure associated with full re-epithelization and hair follicles proliferation was noticed after ten days of treatment. Finally, among the GHME isolated compounds, luteolin 7--D-glucopyranoside exhibited the highest molecular docking score (-9.6 kcal/mol) against matrix metalloproteinase-8 target (MMP-8).