Lupatini Nogueira Rodrigo José, Danopoulos Panagiota, Swikidisa Rosita, Alves Pinheiro Vanessa
Juiz de Fora (SUPREMA), Juiz de Fora, MG, Brazil.
Medisca Pharmaceutique Inc., 6090 Henri-Bourassa Ouest, Saint-Laurent, Canada.
Int J Pharm Compd. 2016 Jul-Aug;20(4):333-337.
The use of natural products in compounded wound care formulas is an exciting avenue to pursue for compounding pharmacists since these natural products may contain compounds that promote healing on their own. The use of these natural extracts as an alternative therapy for wound care may also provide several benefits, such as decreased inflammation, infection, side effects, and treatment costs. Thus far, several studies have demonstrated antimicrobial activity for various natural product extracts, including green propolis and meadowsweet. The antimicrobial properties of these extracts make them particularly interesting for wound care because the healing process is significantly delayed by bacterial infection and colonization at the site of injury. Therefore, to further investigate the antimicrobial properties of green propolis and meadowsweet extracts, we performed minimum inhibitory concentration and minimum bactericidal concentration assays against Staphylococcus aureus, a microorganism known to cause wound infections. The antimicrobial activity of green propolis and meadowsweet extracts was tested in vitro against a standard strain of Staphylococcus aureus in brain heart infusion broth and Mueller-Hinton agar plates. Green propolis extract demonstrated antimicrobial activity against Staphylococcus aureus with a minimum inhibitory concentration of 1.25 mg/mL and a minimum bactericidal concentration of 1.25 mg/mL. In contrast, meadowsweet extract failed to inhibit Staphylococcus aureus growth at the highest concentration tested (30 mg/mL). Green propolis was more effective than meadowsweet extract at inhibiting the growth of Staphylococcus aureus, suggesting that the addition of green propolis extract in wound care formulas might be more beneficial for the treatment of wounds. Therefore, we propose that green propolis extract is a promising natural product for wound care formulations.
对于从事配方调制的药剂师而言，在复合伤口护理配方中使用天然产物是一个值得探索的令人兴奋的途径，因为这些天然产物可能含有自身就能促进愈合的化合物。将这些天然提取物用作伤口护理的替代疗法可能还具有诸多益处，比如减轻炎症、感染、副作用以及降低治疗成本。到目前为止，多项研究已证实各种天然产物提取物具有抗菌活性，包括绿蜂胶和绣线菊。这些提取物的抗菌特性使其在伤口护理方面格外引人关注，因为细菌感染和在损伤部位的定植会显著延迟愈合过程。因此，为了进一步研究绿蜂胶和绣线菊提取物的抗菌特性，我们针对金黄色葡萄球菌进行了最低抑菌浓度和最低杀菌浓度测定，金黄色葡萄球菌是一种已知会导致伤口感染的微生物。在脑心浸液肉汤和穆勒 - 欣顿琼脂平板中，对绿蜂胶和绣线菊提取物的抗菌活性进行了体外测试，以对抗金黄色葡萄球菌的标准菌株。绿蜂胶提取物对金黄色葡萄球菌表现出抗菌活性，最低抑菌浓度为1.25毫克/毫升，最低杀菌浓度为1.25毫克/毫升。相比之下，在测试的最高浓度（30毫克/毫升）下，绣线菊提取物未能抑制金黄色葡萄球菌的生长。在抑制金黄色葡萄球菌生长方面，绿蜂胶比绣线菊提取物更有效，这表明在伤口护理配方中添加绿蜂胶提取物可能对伤口治疗更有益。因此，我们认为绿蜂胶提取物是用于伤口护理配方的一种有前景的天然产物。
