Department of Analytical Chemistry, Institute of Chemical Process Fundamentals of CAS, v.v.i, Rozvojová 135, 165 02, Prague 6, Czech Republic.
Department of Physical Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
Chempluschem. 2022 Aug;87(8):e202200165. doi: 10.1002/cplu.202200165.
Homovanillate (HVA) and vanilmandelate (VMA) are recognized markers of diseases, including neuroblastoma. However, their detection in urine represents a challenging task due to the complexity of the matrix. Here, a design, synthesis and thorough investigation of polymerizable urea-based receptors interacting with HVA and VMA are reported. The selection of receptor with the best anion recognition properties for electrode coating is based on H-NMR and UV-Vis complexation studies. The sensor is prepared by electropolymerization with progress monitoring by cyclic voltammetry. The deposited layer is characterized by IR and scanning electron microscopy. The obtained sensor shows an electrochemical impedance spectroscopy response to VMA with linear range 9.9×10 to 1.2×10 M and LOD of 3.4×10 M. The sensor selectivity was demonstrated by the determination of VMA level in the presence of 16 μM HVA and in artificial urine with and without phosphates, with standard deviations of 0.11, 0.17 and 0.09, respectively.
高香草酸（HVA）和香草扁桃酸（VMA）是公认的疾病标志物，包括神经母细胞瘤。然而，由于基质的复杂性，它们在尿液中的检测是一项具有挑战性的任务。在这里，报道了一种与 HVA 和 VMA 相互作用的可聚合脲基受体的设计、合成和深入研究。基于 H-NMR 和 UV-Vis 络合研究，选择具有最佳阴离子识别性能的受体用于电极涂层。传感器通过循环伏安法进行电聚合，并通过监测进展进行监测。沉积层通过红外和扫描电子显微镜进行表征。所得到的传感器对 VMA 表现出电化学阻抗谱响应，线性范围为 9.9×10 到 1.2×10 M，LOD 为 3.4×10 M。通过在存在 16 μM HVA 和含磷和不含磷的人工尿样中测定 VMA 水平，证明了传感器的选择性，标准偏差分别为 0.11、0.17 和 0.09。
