Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P. R. China.
Anal Chem. 2022 Aug 23;94(33):11643-11649. doi: 10.1021/acs.analchem.2c02207. Epub 2022 Aug 9.
Apoptosis plays a crucial role in the occurrence of cancer and other diseases. Real-time monitoring of the cell apoptosis process has great significance for cell viability and drug screening. Herein, a novel fluorescent probe was constructed based on the fluorescence resonance energy transfer mechanism, which track the sensitivity of polarity changes, as well as detect the drug-induced cell apoptosis process in a dual-color mode. Importantly, the change of cellular microenvironmental polarity makes it possible to dynamically visualize the process of drug-induced cell apoptosis. More significantly, the designed probe targeted the lysosomes in the living cells to give a blue emission, and it accumulated on the plasma membrane to display red fluorescence during the drug-induced cell apoptosis process. Thus, cell viability could be monitored by both the localization and emission colors of the robust probe. We expect that the unique probe can provide a new blueprint for evaluating and screening apoptosis-related drugs.
细胞凋亡在癌症和其他疾病的发生中起着至关重要的作用。实时监测细胞凋亡过程对于细胞活力和药物筛选具有重要意义。在此，我们基于荧光共振能量转移机制构建了一种新型荧光探针，该探针可以跟踪极性变化的灵敏度，并以双色模式检测药物诱导的细胞凋亡过程。重要的是，细胞微环境极性的变化使得动态可视化药物诱导的细胞凋亡过程成为可能。更重要的是，设计的探针靶向活细胞中的溶酶体，发出蓝色发射光，并且在药物诱导的细胞凋亡过程中，它在质膜上积累，显示红色荧光。因此，细胞活力可以通过稳健探针的定位和发射颜色来监测。我们期望该独特的探针能够为评估和筛选与细胞凋亡相关的药物提供新的蓝图。
