Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong, 250022, P. R. China.
Angew Chem Int Ed Engl. 2018 Dec 10;57(50):16506-16510. doi: 10.1002/anie.201811459. Epub 2018 Nov 12.
Mitochondria and nucleus play crucial roles during cell apoptosis process. In this work, a unique fluorescent probe capable of reversible migration between mitochondria and nucleus, as well as detection of cell viability in a dual-color mode is presented. The dual-color probe targets mitochondria in healthy cells, to form aggregates with deep-red emission. It migrates into nucleus and binds to DNA to form monomers with green fluorescence during apoptosis. Interestingly, the migration is reversible dependent on cell viability, which enables the dynamic visualization of apoptosis process. With the probe, mitochondria and nucleus can be visualized in dual colors during apoptosis, and the cell viability could be monitored by the emission color and localization of the probe.
线粒体和细胞核在细胞凋亡过程中起着至关重要的作用。在这项工作中，我们提出了一种独特的荧光探针，它能够在线粒体和细胞核之间可逆迁移，并以双色模式检测细胞活力。该双色探针靶向健康细胞中的线粒体，形成深红光发射的聚集体。在细胞凋亡过程中，它迁移到细胞核并与 DNA 结合形成具有绿色荧光的单体。有趣的是，这种迁移是可逆的，取决于细胞活力，这使得凋亡过程的动态可视化成为可能。利用该探针，在细胞凋亡过程中可以对线粒体和细胞核进行双色可视化，并通过探针的发射颜色和定位来监测细胞活力。
