Sakat Muhammed Sedat, Kılıç Korhan, Sahin Abdulkadir, Ozmen Hilal Kiziltunc, Yıldırım Serkan, Kiziltunc Ahmet, Askin Seda, Saglam Yavuz Selim
Ataturk University, Faculty of Medicine, Department of Otorhinolaryngology, Erzurum, Turkey.
Ataturk University, Faculty of Medicine, Department of Otorhinolaryngology, Erzurum, Turkey.
Arch Oral Biol. 2022 Oct;142:105510. doi: 10.1016/j.archoralbio.2022.105510. Epub 2022 Jul 14.
In this study, we aimed to reveal the effectiveness of Quercetin and Naringenin in preventing radiotherapy-associated submandibular gland injury.
The study was conducted using 48 adult female Sprague Dawley rats. The rats were randomly assigned into six groups of eight animals each. Group 1 represented the control group. The rats received only Naringenin was regarded as Group 2, received only Quercetine was regarded as Group 3. The rats exposed to radiotheraphy at a dose of 15 Gy was regarded as Group 4. Rats in group 5 were received Naringenin at a dose of 50 mg/kg daily for one week prior to radiotheraphy exposition while rats in group 6 was received Quercetine at a dose of 50 mg/kg daily for one week prior to radiotheraphy. Rats were sacrificed after radiotheraphy and submandibular glands were dissected for biochemical and immunohistochemical evaluations.
Quercetin and Naringenin were found to have protective effect against radiation-induced damage. Naringenin and Quercetin increased the levels of Superoxide dismutase, Catalase, Glutathione Peroxidase, Glutathione and Total antioxidant status while decreasing the levels of Myeloperoxidase and Total oxidant status. Also, these agents inhibited the expression of Tumor Necrosis Factor-α and 8-hydroxy 2-deoxyguanosine immunohistochemically.
With their powerful antioxidant and anti-inflammatory effects, Naringenin and Quercetin exhibit histopathological, immunochemical, and biochemical protection against radiation-related submandibular gland injury. In addition, Quercetin was found to be superior to Naringenin in terms of this efficacy.
在本研究中，我们旨在揭示槲皮素和柚皮素在预防放疗相关下颌下腺损伤方面的有效性。
该研究使用了48只成年雌性Sprague Dawley大鼠。大鼠被随机分为六组，每组八只动物。第1组为对照组。仅接受柚皮素的大鼠被视为第2组，仅接受槲皮素的大鼠被视为第3组。接受15 Gy剂量放疗的大鼠被视为第4组。第5组大鼠在放疗前一周每天接受50 mg/kg剂量的柚皮素，而第6组大鼠在放疗前一周每天接受50 mg/kg剂量的槲皮素。放疗后处死大鼠，解剖下颌下腺进行生化和免疫组织化学评估。
发现槲皮素和柚皮素对辐射诱导的损伤具有保护作用。柚皮素和槲皮素提高了超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽水平和总抗氧化状态，同时降低了髓过氧化物酶水平和总氧化状态。此外，这些药物在免疫组织化学上抑制了肿瘤坏死因子-α和8-羟基-2'-脱氧鸟苷的表达。
柚皮素和槲皮素具有强大的抗氧化和抗炎作用，对辐射相关的下颌下腺损伤具有组织病理学、免疫化学和生化保护作用。此外，在这种疗效方面，发现槲皮素优于柚皮素。
