School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, Shandong Province, China.
Department of Food Science & Technology, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Food Chem. 2022 Dec 15;397:133838. doi: 10.1016/j.foodchem.2022.133838. Epub 2022 Jul 30.
The bioelectronic tongues based on taste receptors have been emerging with human-like taste perception. However, the practical applications of the receptor-based biosensors were restricted by their narrow and low dynamic ranges. Here, a novel immobilization strategy based on AuNPs@ZIF-8/TiC MXene was developed to immobilize the umami ligand binding domain (T1R1-VFT), to fabricate an umami biosensor for umami substances detection. Through the synergic effect of AuNPs@ZIF-8 and TiC MXene, the capacity to load T1R1-VFT was effectively increased, and the response signal was also amplified by approximately 3 times. The proposed biosensor showed an ultrawide dynamic range of 10-10 M, and a high upper limit of detection, which was closer to the human taste threshold and suitable for detecting foods rich in umami substances. Additionally, the biosensor was successfully applied to detect real samples and analyze the synergistic effects of binary umami substances.
基于味觉受体的生物电子舌具有类似人类的味觉感知能力。然而，受体生物传感器的实际应用受到其狭窄和低动态范围的限制。在这里，开发了一种基于 AuNPs@ZIF-8/TiC MXene 的新型固定化策略，用于固定鲜味配体结合结构域（T1R1-VFT），以制备用于鲜味物质检测的鲜味生物传感器。通过 AuNPs@ZIF-8 和 TiC MXene 的协同作用，有效增加了 T1R1-VFT 的装载能力，响应信号也放大了约 3 倍。所提出的生物传感器具有超宽的 10-10 M 动态范围和较高的检测上限，更接近人类的味觉阈值，适合检测富含鲜味物质的食品。此外，该生物传感器成功应用于检测实际样品，并分析二元鲜味物质的协同作用。
