College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Agricultural Equipment in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen 518000, PR China; Key Laboratory of Aquaculture Facilities Engineering, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China.
College of Engineering, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Agricultural Equipment in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China; Key Laboratory of Aquaculture Facilities Engineering, Ministry of Agriculture and Rural Affairs, Wuhan 430070, PR China.
Food Chem. 2023 Oct 15;423:136233. doi: 10.1016/j.foodchem.2023.136233. Epub 2023 Apr 23.
Umami substances can provide a palatable flavour for food. In this study, an electrochemical impedimetric biosensor was developed for detecting umami substances. This biosensor was fabricated by immobilising T1R1 onto AuNPs/reduced graphene oxide/chitosan which was in advance electro-deposited onto a glassy carbon electrode. The evaluation by the electrochemical impedance spectrum method showed that the T1R1 biosensor performed well with low detection limits and wide linear ranges. Under the optimised incubation time (60 s), the electrochemical response was linearly related to the concentrations of the detected targets monosodium glutamate and inosine-5'-monophosphate within their respective linear range of 10 to 10 M and 10 to 10 M. The low detection limit of monosodium glutamate and inosine-5'-monophosphate was 10 M and 10 M, respectively. Moreover, the T1R1 biosensor exhibited high specificity to umami substances even in the real food sample. The developed biosensor still retained 89.24% signal intensity after 6-day storage, exhibiting a desirable storability.
鲜味物质可为食物提供可口的味道。在这项研究中，我们开发了一种电化学阻抗生物传感器来检测鲜味物质。该生物传感器通过将 T1R1 固定在 AuNPs/还原氧化石墨烯/壳聚糖上制备而成，该复合物预先通过电沉积到玻碳电极上。电化学阻抗谱法的评估表明，T1R1 生物传感器具有良好的性能，检测限低，线性范围宽。在优化的孵育时间（60 秒）下，电化学响应与检测目标谷氨酸单钠和肌苷-5'-单磷酸的浓度在各自的 10 到 10 M 和 10 到 10 M 的线性范围内呈线性关系。谷氨酸单钠和肌苷-5'-单磷酸的检测限分别为 10 M 和 10 M。此外，T1R1 生物传感器即使在真实食物样品中也对鲜味物质表现出高特异性。在 6 天的储存后，开发的生物传感器仍然保留了 89.24%的信号强度，表现出良好的储存稳定性。
