Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
Poult Sci. 2022 Oct;101(10):102057. doi: 10.1016/j.psj.2022.102057. Epub 2022 Jul 14.
Bacterial antimicrobial resistance (AMR) continues to develop, with the horizontal transfer of antibiotic resistance genes (ARGs) through plasmids playing a major role. Recently, the antimicrobial resistance of R. anatipestifer has become increasingly severe, jeopardizing the development of the poultry industry. In this study, we used PromethION to determine the whole genome sequence of R. anatipestifer RCAD0416, a multidrug-resistant isolate from China. We detected a plasmid in the isolate. We named the plasmid pRCAD0416RA-1; the plasmid was 37356 bp in size with 36 putative open reading frames and included the bla, floR, tet(X), ermF, ereD, and AadS resistance genes. Most resistance genes might be obtained from R. anatipestifer HXb2. Mobile elements and floR might be transmitted by plasmid pB18-2 from Acinetobacter indicus, and the ICEPg6Chn1 mobile elements can be transmitted from Proteus genomosp. The plasmid pRCAD0416RA-1 was transferred to Escherichia coli K-12 × 7232 via electroporation. Subsequent antimicrobial sensitivity tests (AST) showed a noticeable levels of antimicrobial resistance to β-lactams (4-8 fold), tigecycline (8 fold), and florfenicol (8 fold). These types of antibiotics are in common clinical use. The purpose of this article is to elucidate the basic characteristics of pRCAD0416RA-1 and the level of resistance mediated by bla, floR, and tet(X).
细菌对抗生素的耐药性（AMR）不断发展，通过质粒进行抗生素耐药基因（ARGs）的水平转移起着主要作用。最近，鸭疫里默氏杆菌的抗药性变得越来越严重，威胁到家禽产业的发展。在本研究中，我们使用 PromethION 来确定来自中国的多药耐药分离株 R. anatipestifer RCAD0416 的全基因组序列。我们在分离株中检测到一个质粒。我们将该质粒命名为 pRCAD0416RA-1；该质粒大小为 37356 bp，包含 36 个推定的开放阅读框，其中包括 bla、floR、tet(X)、ermF、ereD 和 AadS 耐药基因。大多数耐药基因可能来自 R. anatipestifer HXb2。移动元件和 floR 可能通过来自不动杆菌的质粒 pB18-2 传播，而 ICEPg6Chn1 移动元件可以从基因组型 Proteus 传播。通过电穿孔将质粒 pRCAD0416RA-1 转移到大肠杆菌 K-12 × 7232。随后的抗菌药物敏感性测试（AST）显示对β-内酰胺类（4-8 倍）、替加环素（8 倍）和氟苯尼考（8 倍）的抗菌药物有明显的耐药性。这些类型的抗生素在临床上经常使用。本文的目的是阐明 pRCAD0416RA-1 的基本特征以及 bla、floR 和 tet(X)介导的耐药水平。
