de Oliveira Rodrigo Cardoso, Mendonça Carlos Miguel Nobrega, Verissimo Nathalia Vieira, de Almeida Sonia Regina Yokomizo, Correa Benedito, Watanabe Ii-Sei, de Azevedo Pamela Oliveira de Souza, Oliveira Ricardo Pinheiro de Souza
Laboratory of Microbial Biomolecules, Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, Brazil.
Department of Anatomy, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
J Appl Microbiol. 2022 Nov;133(5):3020-3029. doi: 10.1111/jam.15746. Epub 2022 Aug 10.
This study aims to demonstrate the potential of the lactic acid bacteria (LAB) Pediococcus pentosaceus LBM18 against the mycotoxin-producing Alternaria alternata TEF-1A and highlight its application as an effective grain silage inoculant to control mycotoxin contamination.
The antifungal properties of Ped. pentosaceus lyophilized (PPL) were assessed by evaluating its effect on A. alternata TEF-1A grown in a corn silage-based medium, which included morphological changes by Scanning Electron Microscopy (SEM) observations, growth rate, conidia production assays, and inhibition of Tenuazonic acid (TeA) production by high-performance liquid chromatography (HPLC-MS/MS) analyses. Furthermore, TeA biosynthesis was monitored for changes at the molecular level by PKS gene expression. The growth and sporulation processes of A. alternata TEF-1A were affected by Ped. pentosaceus LBM18 in a concentration-dependent manner. Moreover, a significant inhibition of TeA production (74.3%) and the transcription level of the PKS gene (42.9%) was observed.
Ped. pentosaceus is one of the promising LAB to be applied as an inoculant for corn silage preservation, aiming to inhibit mycotoxigenic fungi growth and their mycotoxin production.
Ped. pentosaceus could be used as an inoculant to reduce fungal and mycotoxins contamination in grain silage production.
本研究旨在证明戊糖片球菌LBM18对产真菌毒素的链格孢菌TEF - 1A的抑制潜力，并突出其作为一种有效的谷物青贮接种剂在控制真菌毒素污染方面的应用。
通过评估戊糖片球菌冻干物（PPL）对在基于玉米青贮的培养基中生长的链格孢菌TEF - 1A的影响，来评估其抗真菌特性，这些影响包括通过扫描电子显微镜（SEM）观察形态变化、生长速率、分生孢子产生测定以及通过高效液相色谱（HPLC - MS/MS）分析对细交链格孢菌酮酸（TeA）产生的抑制作用。此外，通过聚酮合酶（PKS）基因表达在分子水平监测TeA生物合成的变化。链格孢菌TEF - 1A的生长和孢子形成过程受到戊糖片球菌LBM18的浓度依赖性影响。此外，观察到TeA产量显著抑制（74.3%）和PKS基因转录水平显著抑制（42.9%）。
戊糖片球菌是有望用作玉米青贮保存接种剂的乳酸菌之一，旨在抑制产真菌毒素真菌的生长及其真菌毒素的产生。
戊糖片球菌可用作接种剂，以减少谷物青贮生产中的真菌和真菌毒素污染。
