Xu Xi, Guo Lifeng, Yang Chunbo, Teng Haolin, Shen Guijin, Wang Shuo, Zhao Junlei, Yang Xilang, Zhang Li, Wang Xiangjing, Zhao Junwei, Xiang Wensheng
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. China.
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, P.R. China.
Plant Dis. 2023 Mar;107(3):794-801. doi: 10.1094/PDIS-05-22-1254-RE. Epub 2023 Mar 3.
Winter jujube originated from China and had an extremely high nutritional value. In 2021, symptomatic winter jujube fruits were collected from eight locations in Zhanhua District of Binzhou City, Shandong Province. In total, 108 fungal isolates were obtained and grouped into 11 species based on morphological characteristics and multilocus phylogenetic analysis, including (43.52%), (20.37%), (12.03%), (7.41%), (4.63%), (3.70%), sp. (2.78%), (2.78%), (1.85%), (0.93%), and (0.93%). All fungal isolates obtained in this study showed aggressiveness on detached winter jujube fruits except and isolates, of which was the most virulent, while isolates, which have been considered the major pathogen of winter jujube fruit rot, showed a relatively low-level virulence in this study. Furthermore, , , , and an unclassified species were first reported as causal agents of winter jujube fruit rot. The typical symptoms of winter jujube fruit rot observed in this study could be distinguished into two types. , , . , sp., , and isolates caused reddish brown to dark gray lesions on the peel, while , , and isolates caused peel and pulp decay, resulting in red to reddish brown and water-soaked lesions. In addition, haplotype analysis of isolates obtained in this study and validly published articles showed that there were 11 haplotypes worldwide; the isolates obtained in the current study were grouped into three haplotypes (Hap 1, Hap 2, and Hap 11), and two of them (Hap 2 and Hap 11) were confirmed as new haplotypes.
冬枣原产于中国，具有极高的营养价值。2021年，从山东省滨州市沾化区的8个地点采集了有症状的冬枣果实。共获得108株真菌分离物，根据形态特征和多位点系统发育分析将其分为11个种，包括（43.52%）、（20.37%）、（12.03%）、（7.41%）、（4.63%）、（3.70%）、sp.（2.78%）、（2.78%）、（1.85%）、（0.93%）和（0.93%）。本研究中获得的所有真菌分离物，除了和分离物外，对离体冬枣果实均表现出侵染性，其中是最具毒性的，而被认为是冬枣果实腐烂主要病原菌的分离物，在本研究中表现出相对较低的毒性。此外，、、和一个未分类的种首次被报道为冬枣果实腐烂的病原菌。本研究中观察到的冬枣果实腐烂的典型症状可分为两种类型。、、。、sp.、和分离物在果皮上引起红棕色至深灰色病斑，而、和分离物引起果皮和果肉腐烂，导致红色至红棕色和水渍状病斑。此外，对本研究中获得的分离物和有效发表文章进行的单倍型分析表明，全球有11种单倍型；本研究中获得的分离物分为三种单倍型（单倍型1、单倍型2和单倍型11），其中两种（单倍型2和单倍型11）被确认为新的单倍型。
