Overexpression of CaMYB78 transcription factor enhances resistance response in chickpea against Fusarium oxysporum and negatively regulates anthocyanin biosynthetic pathway.

Chickpea, an important grain legume, suffers from considerable loss of yield due to Fusarium wilt disease. Inaccessibility of resistant gene pool among cultivars and lack of report of resistance, genes from alien sources have been the major constraints for resistance development in this valuable crop. However, along with some other transcription factors, MYB78 was significantly upregulated during chickpea-Fusarium interplay in resistant chickpea genotype. Being a highly recalcitrant species, the transformation of this important crop remained non-reproducible until recently. Following a tissue culture independent plumular meristem transformation protocol, introgression of CaMYB78 TF finally became feasible in chickpea. The overexpressed plants developed resistance against the pathogen but the anthocyanin production in transformed flowers was perturbed. In silico analyses of the anthocyanin biosynthetic key gene promoters reported the occurrence of multiple MYB-binding cis elements. Detailed molecular analyses establish the differential regulatory roles of CaMYB78, resistance response against Foc1 on one hand and suppression of pigmentation during flower development on the other, which is an innovative finding of its kind.