Vasini Brenda, Farace Pablo, Ariel Amadio, Cirone Karina, Mendez Laura, Morsella Claudia, Fresia Pablo, Iraola Gregorio, Gioffré Andrea, Paolicchi Fernando
Laboratorio de Bacteriología-Grupo de Sanidad Animal, Unidad Integrada INTA-Universidad Nacional de Mar del Plata-Balcarce, Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina.
Vet Res Commun. 2022 Dec;46(4):1121-1129. doi: 10.1007/s11259-022-09983-8. Epub 2022 Aug 11.
Paratuberculosis is a worldwide chronic enteric disease of ruminants, caused by Mycobacterium avium subsp. paratuberculosis (MAP). While MAP has been widely investigated all around the world, little is known about the different strains that circulate in each country. This study describes the genetic diversity of MAP isolates from different bovine and deer herds from Argentina, analyzed by Multiple-Locus Variable number tandem repeat Analysis (MLVA), as well as the phylogenetic relatedness between geographically distant isolates through Whole Genome Sequencing (WGS) and core-genome analysis. A total of 90 MAP isolates were analyzed. The results showed seven different MLVA genotypes, with almost 75% of them belonging to pattern INMV 1, described in all the herds studied. WGS results suggested the presence of a common INMV 1 strain circulating throughout the country. Our results allow confirming the coexistence of different strains in time and space and the mixed infections identified in some animals. These observations suggest the absence of animal monitoring prior to introduction to the herds and the need for a control program in the country. This study represents the first to report WGS of MAP strains in Argentina.
副结核病是一种在全球范围内影响反刍动物的慢性肠道疾病，由鸟分枝杆菌副结核亚种（MAP）引起。尽管MAP在全球范围内已得到广泛研究，但对于每个国家流行的不同菌株却知之甚少。本研究描述了通过多位点可变数目串联重复序列分析（MLVA）对来自阿根廷不同牛群和鹿群的MAP分离株的遗传多样性，以及通过全基因组测序（WGS）和核心基因组分析对地理上相距遥远的分离株之间的系统发育相关性进行的分析。总共分析了90株MAP分离株。结果显示有七种不同的MLVA基因型，其中近75%属于模式INMV 1，在所研究的所有畜群中均有描述。WGS结果表明，全国存在一种共同的INMV 1菌株在传播。我们的结果证实了不同菌株在时间和空间上的共存以及在一些动物中发现的混合感染。这些观察结果表明，在引入畜群之前缺乏动物监测，并且该国需要一个控制计划。本研究是阿根廷首次报道MAP菌株的WGS。
