Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 1655/76, Pilsen, Czech Republic.
Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, Pilsen, Czech Republic.
In Vitro Cell Dev Biol Anim. 2022 Sep;58(8):643-657. doi: 10.1007/s11626-022-00713-7. Epub 2022 Aug 10.
Dermal fibroblasts (DF) constitute one of key cells involved in wound healing. However, the functions they perform in wound conditions remain poorly understood. This study involved exposing DF to low nutrition and to low nutrition + LPS for 5 d as conditions representing the wound. Although DF exhibited increasing metabolic activity in time under all conditions including control, the proliferation did not change in both low nutrition and low nutrition + LPS. Only the low nutrition + LPS was found to potentiate the migration and pro-inflammatory phenotype (IL6 release) of DF. The potential of DF to contract collagen hydrogel declined only under low nutrition as a consequence of low cell number. The expression of α-SMA was reduced under both conditions independently of the cell number. The remodeling capability of DF was affected under both conditions as documented by the enhanced MMP2 activity. Finally, the production of collagen type I was not affected by either condition. The study shows that low nutrition as the single factor is able to delay the healing process. Moreover, the addition of the mild pro-inflammatory stimulus represented by LPS may amplify the cell response in case of decreased α-SMA expression or excite DF to produce IL6 impairing the healing process.
真皮成纤维细胞（DF）是参与伤口愈合的关键细胞之一。然而，它们在伤口条件下的功能仍知之甚少。本研究将 DF 暴露于低营养和低营养+LPS 中 5 天，作为代表伤口的条件。尽管 DF 在包括对照在内的所有条件下的代谢活性随时间增加，但在低营养和低营养+LPS 中增殖没有变化。只有低营养+LPS 被发现增强了 DF 的迁移和促炎表型（IL6 释放）。由于细胞数量较少，只有在低营养下，DF 收缩胶原水凝胶的能力才会下降。α-SMA 的表达在两种条件下均降低，而与细胞数量无关。DF 的重塑能力受到两种条件的影响，这一点通过增强的 MMP2 活性得到证明。最后，胶原 I 型的产生不受任何条件的影响。该研究表明，低营养作为单一因素能够延缓愈合过程。此外，添加由 LPS 代表的轻度促炎刺激物可能会放大细胞反应，降低 α-SMA 表达，或刺激 DF 产生 IL6，从而损害愈合过程。
