Department of Pharmaceutical Botany, Faculty of Pharmacy, Süleyman Demirel University, Isparta, Turkey.
Department Pharmaceutical Botany, Faculty of Pharmacy, İstinye University, İstanbul, Turkey.
BMC Complement Med Ther. 2022 Aug 10;22(1):215. doi: 10.1186/s12906-022-03693-z.
Cordia myxa L. (Boraginaceae) is widely distributed in tropical regions and it's fruits, leaves and stem bark have been utilized in folk medicine for treating trypanosomiasis caused by Trypanosoma cruzi. A population-based study showed that T. cruzi infection is associated with cognitive impairments. Therefore, if C. myxa has ameliorating activities on cognitive function, it would be useful for both T. cruzi infection and cognitive impairments.
In this study, we evaluated the effects of an ethanol extract of leaves of C. myxa (ELCM) on memory impairments and sensorimotor gating deficits in mice. The phosphorylation level of protein was observed by the Western blot analysis.
The administration of ELCM significantly attenuated scopolamine-induced cognitive dysfunction in mice, as measured by passive avoidance test and novel object recognition test. Additionally, in the acoustic startle response test, we observed that the administration of ELCM ameliorated MK-801-induced prepulse inhibition deficits. We found that these behavioral outcomes were related with increased levels of phosphorylation phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt) and glycogen synthase kinase 3 beta (GSK-3β) in the cortex and extracellular signal-regulated kinase (ERK) and cAMP response element-binding protein (CREB) in the hippocampus by western blot analysis.
These results suggest that ELCM would be a potential candidate for treating cognitive dysfunction and sensorimotor gating deficits observed in individuals with neurodegenerative diseases.
黄麻（紫草科）广泛分布于热带地区，其果实、叶和茎皮在民间医学中被用于治疗由克氏锥虫引起的锥虫病。一项基于人群的研究表明，克氏锥虫感染与认知障碍有关。因此，如果黄麻具有改善认知功能的作用，那么它对于克氏锥虫感染和认知障碍都将是有用的。
在这项研究中，我们评估了黄麻叶乙醇提取物（ELCM）对小鼠记忆障碍和感觉运动门控缺陷的影响。通过 Western blot 分析观察蛋白质的磷酸化水平。
ELCM 的给药显著减轻了东莨菪碱诱导的小鼠认知功能障碍，如被动回避测试和新物体识别测试所示。此外，在听觉惊跳反应测试中，我们观察到 ELCM 改善了 MK-801 诱导的前脉冲抑制缺陷。我们发现这些行为结果与皮层中磷酸化磷脂酰肌醇 3-激酶（PI3K）、蛋白激酶 B（Akt）和糖原合成酶激酶 3β（GSK-3β）水平的增加以及海马中的细胞外信号调节激酶（ERK）和 cAMP 反应元件结合蛋白（CREB）有关Western blot 分析。
这些结果表明，ELCM 可能是治疗神经退行性疾病患者中观察到的认知功能障碍和感觉运动门控缺陷的潜在候选药物。
