Chen Yongjun, Yang Xuefeng, Liu Jian, Zhang Dandan, He Jun, Tang Liang, Li Jianming, Xiang Qin
Department of Neurology, Nanhua Affiliated Hospital, Hengyang Medical College, University of South China, Hengyang, China.
Department of Gastroenterology, Nanhua Affiliated Hospital, Hengyang Medical College, University of South China, Hengyang, China.
Nucleosides Nucleotides Nucleic Acids. 2023;42(2):105-118. doi: 10.1080/15257770.2022.2109170. Epub 2022 Aug 10.
Nucleic acid aptamers are developed from a pool of random oligonucleotide libraries with an selection through systematic evolution of ligands via exponential enrichment (SELEX) process, which are capable of specific and high-affinity molecular binding against targets. The receptor-binding domain (RBD) of spike protein from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is involved in the early stages of viral infection, is a promising target for aptamer selection. Currently, there are no effective approaches to prevent virus from spreading. In this study, a new ssDNA aptamer RBD/S-A1 binding to the RBD of spike protein from SARS-CoV-2 with high affinity (K=1.74 ± 0.2 nM) and low cross-binding activity was selected and evaluated. Although aptamers targeting the RBD of spike protein from SARS-CoV-2 have been described in a handful of previous studies, the RBD/S-A1 aptamer identified in this work may be considered as a potential supplementation for the current diagnosis and research of coronavirus SARS-CoV-2.
核酸适配体是从随机寡核苷酸文库中筛选出来的，通过指数富集配体系统进化（SELEX）过程进行筛选，能够与靶标进行特异性高亲和力分子结合。严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突蛋白的受体结合域（RBD）参与病毒感染的早期阶段，是适配体筛选的一个有前景的靶标。目前，尚无有效的方法阻止病毒传播。在本研究中，筛选并评估了一种新的单链DNA适配体RBD/S-A1，它能与SARS-CoV-2刺突蛋白的RBD高亲和力结合（K=1.74±0.2 nM）且交叉结合活性低。尽管之前有少数研究报道了靶向SARS-CoV-2刺突蛋白RBD的适配体，但本研究中鉴定出的RBD/S-A1适配体可被视为当前冠状病毒SARS-CoV-2诊断和研究的潜在补充。
