Zhen Zicheng, Li Minghao, Zhong Muyan, Liu Jiaqi, Huang Wendu, Ye Liqun
Zhongshan School of Medicine, Sun Yat-sen University Guangzhou 510060, Guangdong, China.
The Second Clinical Medical School, Guangdong Medical University Dongguan 523808, Guangdong, China.
Int J Clin Exp Pathol. 2022 Jul 15;15(7):258-271. eCollection 2022.
TMEM204 (Transmembrane Protein 204) is a member of the TMEM family that regulates cell function and angiogenesis. Previous studies showed that TMEM204 is related to pancreatic cancer, but its roles in other cancers remain unknown. To reveal this relationship, we conducted a pan-cancer analysis by several online databases. The expression of TMEM204 was analyzed by Oncomine and Tumor Immune Estimation Resource2.0 (TIMER2.0). The prognostic potential of TMEM204 was evaluated by the GEPIA2, UALCAN, and Oncolnc. The methylation level of gene expression was analyzed by UALCAN, and the relationship between cancer and immune invasion was displayed by TIMER2.0. The Protein-Protein Interactions Network and functional analysis of TMEM204 and its related genes were conducted by STRING and Webgestalt. We found that TMEM204 expression was up-regulated and correlated with prognosis in multiple cancers. In liver hepatocellular carcinoma (LIHC), high TMEM204 expression was associated with a good prognosis, and with high infiltrating levels of CD8 T and CD4 T cells, macrophages, neutrophils, and myeloid dendritic cells. In addition, the methylation level in LIHC was higher than in normal tissues. p53 signaling pathway and Fanconi anemia pathway were implicated by KEGG pathway analysis. These results indicate that TMEM204 is associated with the prognosis, methylation, and immune invasion of cancers, especially LIHC. TMEM204 may act as a prognostic marker of LIHC and its role in other cancers should be studied.
跨膜蛋白204（TMEM204）是跨膜蛋白（TMEM）家族的成员之一，可调节细胞功能和血管生成。先前的研究表明TMEM204与胰腺癌有关，但其在其他癌症中的作用仍不清楚。为了揭示这种关系，我们通过几个在线数据库进行了泛癌分析。利用Oncomine和肿瘤免疫评估资源2.0（TIMER2.0）分析TMEM204的表达。通过GEPIA2、UALCAN和Oncolnc评估TMEM204的预后潜力。利用UALCAN分析基因表达的甲基化水平，并通过TIMER2.0展示癌症与免疫浸润之间的关系。通过STRING和Webgestalt对TMEM204及其相关基因进行蛋白质-蛋白质相互作用网络分析和功能分析。我们发现TMEM204在多种癌症中的表达上调且与预后相关。在肝细胞癌（LIHC）中，高表达的TMEM204与良好的预后相关，并且与CD8 T细胞、CD4 T细胞、巨噬细胞、中性粒细胞和髓样树突状细胞的高浸润水平有关。此外，LIHC中的甲基化水平高于正常组织。KEGG通路分析表明p53信号通路和范可尼贫血通路参与其中。这些结果表明TMEM204与癌症尤其是LIHC的预后、甲基化和免疫浸润有关。TMEM204可能作为LIHC的预后标志物，其在其他癌症中的作用有待进一步研究。
