Department of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil.
Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil.
J Biomed Mater Res B Appl Biomater. 2023 Jan;111(1):151-160. doi: 10.1002/jbm.b.35141. Epub 2022 Aug 11.
The development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol-gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro-Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal-Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering.
多年来，与聚合物相关的纳米级生物材料的发展一直在增长，因为它们在生物系统中的应用具有重要的结构特征。本研究旨在制备和测试由聚乳酸（PLA）纤维组成的聚合物支架，该支架与掺有治疗离子的 58S 生物玻璃结合使用，用于组织工程。通过溶胶-凝胶法获得了三种 58S 生物玻璃，分别为纯 58S 生物玻璃和掺杂 5%锶和钴离子的 58S 生物玻璃。使用 7%PLA 的溶液作为对照，并添加了三种不同的生物玻璃，即 4%的 58S 生物玻璃（PLA-BG）、4%掺锶的生物玻璃（PLA-BGSr）和 4%掺钴的生物玻璃（PLA-BGCo）。通过静电纺丝工艺制备支架，并对其进行化学和形态学表征。通过体外测试，使用来自 9 只大鼠股骨的间充质细胞培养物进行，在补充有总培养物的成骨培养基中生长。在诱导成骨细胞分化后，进行细胞活力、碱性磷酸酶活性、总蛋白含量定量和矿化结节测试。使用 Shapiro-Wilk 检验（纳米纤维直径和生物测定）对正态分布进行分析。使用 Kruskal-Wallis 非参数检验（p=0.05）比较数据。结果表明，所制备的生物玻璃不含硝酸盐、氯化物和纳米级物质，并且治疗离子有效掺入其结构中。所有材料均显示出>70%的细胞活力、总蛋白产量和碱性磷酸酶活性。可以开发出与掺有治疗离子的 58S 生物玻璃结合的聚乳酸支架，而无细胞毒性。支架的特性似乎支持其在骨组织工程中的应用。
