Department of Biology, Basic Science Faculty, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Cellular and Molecular Biology, Faculty of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Mol Biol Rep. 2024 Jul 23;51(1):838. doi: 10.1007/s11033-024-09763-4.
Bioglass materials have gained significant attention in the field of tissue engineering due to their osteoinductive and biocompatible properties that promote bone cell differentiation. In this study, a novel composite scaffold was developed using a sol-gel technique to combine bioglass (BG) 58 S with a poly L-lactic acid (PLLA).
The physiochemical properties, morphology, and osteoinductive potential of the scaffolds were investigated by X-ray diffraction analysis, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The results showed that the SiO-CaO-PO system was successfully synthesized by the sol-gel method. The PLLA scaffolds containing BG was found to be osteoinductive and promoted mineralization, as demonstrated by calcium deposition assay, upregulation of alkaline phosphatase enzyme activity, and Alizarin red staining data.
These in vitro studies suggest that composite scaffolds incorporating hBMSCs are a promising substitute material to be implemented in bone tissue engineering. The PLLA/BG scaffolds promote osteogenesis and support the differentiation of bone cells, such as osteoblasts, due to their osteoinductive properties.
由于生物玻璃材料具有骨诱导性和生物相容性，能促进成骨细胞分化，因此在组织工程领域受到了广泛关注。本研究采用溶胶-凝胶技术，将生物玻璃 58S 与聚 L-乳酸（PLLA）相结合，开发了一种新型复合支架。
通过 X 射线衍射分析、扫描电子显微镜和傅里叶变换红外光谱对支架的物理化学性质、形态和骨诱导潜能进行了研究。结果表明，成功地通过溶胶-凝胶法合成了 SiO-CaO-PO 体系。含有 BG 的 PLLA 支架具有骨诱导性，并促进矿化，通过钙沉积测定、碱性磷酸酶酶活性的上调和茜素红染色数据得到证实。
这些体外研究表明，复合支架结合 hBMSCs 是一种很有前途的替代材料，可用于骨组织工程。由于其骨诱导特性，PLLA/BG 支架能促进成骨，并支持成骨细胞（如成骨细胞）的分化。
