Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, China.
Shanghai University of Sport, China.
Folia Neuropathol. 2022;60(2):228-236. doi: 10.5114/fn.2022.118187.
The diagnosis of post-stroke cognitive impairment (PSCI) mainly depends on neuro-psychological evaluation. It still lacks a sensitive and objective diagnostic biomarker. MicroRNAs (miRNAs, miRs) are novel and potential disease biomarkers. Our aim was to detect which specific miRNA is a good diagnostic biomarker for PSCI.
There were 77 first-ever stroke patients enrolled. Blood samples were collected at 14 days after stroke. Level of serum miR-21, miR-124, miR-132, and miR-200b were determined by quantitative polymerase chain reaction. Mini-mental state examination (MMSE) scale was used to measure the cognitive function of patients. Factional anisotropy (FA) score of diffusion tensor imaging was applied to detect the alteration of white matter. In addition, the relationship between miRNA level and cognitive status was further explored by correlation analysis.
Finally, 45 PSCI and 32 post-stroke cognitive normality (PSCN) patients were enrolled. The expression of miR-21, miR-132, and miR-200b in PSCI patients was higher than in PSCN patients. In particular, the miR-21 level was substantially correlated with MMSE scores (r = 0.752, p < 0.001) and FA value (r = 0.636, p < 0.001). Additionally, the diagnostic performance of miR-21 alone or the combination of miR-21 and FA values performed well.
The miR-21 alone or combination of miR-21 and FA values are valuable diagnostic biomarkers in discriminating PSCI from PSCN.
脑卒中后认知障碍（PSCI）的诊断主要依赖于神经心理学评估，目前仍缺乏敏感和客观的诊断生物标志物。microRNAs（miRNAs，miRs）是新型潜在疾病生物标志物。本研究旨在检测哪种特定 miRNA 是 PSCI 的良好诊断生物标志物。
共纳入 77 例首次脑卒中患者，在脑卒中后 14 天采集血样，采用实时定量聚合酶链反应（qPCR）检测血清 miR-21、miR-124、miR-132 和 miR-200b 水平，采用简易精神状态检查量表（MMSE）评估患者认知功能，应用弥散张量成像的分数各向异性（FA）评分检测白质改变，进一步通过相关性分析探讨 miRNA 水平与认知状态的关系。
最终纳入 45 例 PSCI 和 32 例脑卒中后认知正常（PSCN）患者。PSCI 患者的 miR-21、miR-132 和 miR-200b 表达高于 PSCN 患者，miR-21 水平与 MMSE 评分（r = 0.752，p < 0.001）和 FA 值（r = 0.636，p < 0.001）呈显著相关。此外，miR-21 单独或 miR-21 与 FA 值联合的诊断性能较好。
miR-21 单独或联合 FA 值可作为鉴别 PSCI 与 PSCN 的有价值的诊断生物标志物。
