Soriano Leonardo, Omar Ahmad A, Martinelli Adriana Pinheiro
University of São Paulo, CENA, Piracicaba, SP, Brazil.
Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Methods Mol Biol. 2022;2527:111-126. doi: 10.1007/978-1-0716-2485-2_9.
Protoplasts are an attractive explant source for biotechnological tools widely used on citrus genetic improvement, such as somatic hybridization and direct genetic transformation. These delicate and responsive materials are subjected to cell proliferation induction and differentiation of somatic embryos which further regenerate into entire plants. The isolation of viable protoplasts followed by regeneration of plants through somatic embryogenesis is an important methodology for breeding applications. The methods presented here can also be used as a reference for protoplast work in other species, followed by protocol optimization for different species/genotypes.
原生质体是用于柑橘遗传改良的生物技术工具（如体细胞杂交和直接遗传转化）的一种有吸引力的外植体来源。这些脆弱且敏感的材料会经历体细胞胚胎的细胞增殖诱导和分化，进而再生为完整植株。分离出有活力的原生质体，随后通过体细胞胚胎发生再生植株，是育种应用中的一项重要方法。这里介绍的方法也可作为其他物种原生质体研究的参考，之后可针对不同物种/基因型对方案进行优化。
