来自未成熟橄榄合子胚的体细胞胚胎发生

Somatic Embryogenesis from Immature Olive Zygotic Embryos.

作者信息

Trabelsi El Bahri, Jedidi Emna

机构信息

Tunis El Manar University, Tunis, Tunisia.

National Agricultural Research Institute of Tunisia Laboratory of Horticulture, University of Carthage, Tunis, Tunisia.

出版信息

Methods Mol Biol. 2022;2527:133-141. doi: 10.1007/978-1-0716-2485-2_11.

DOI:10.1007/978-1-0716-2485-2_11

PMID:35951189

Abstract

The effects of plant growth regulators (PGRs) and the explant type on the embryogenesis and plant regeneration of olive (Olea europaea L. ssp europaea var. sativa) cv. "Chetoui" were studied using immature zygotic embryos. Embryogenic callus induction was achieved on OMc medium supplemented with different concentrations of BAP and NAA at low levels. Immature zygotic embryos as juvenile tissues are competent for somatic embryogenesis independently of PGRs supplementation. Repetitive somatic embryogenesis was obtained on PGRs-free media or at low concentrations in the dark.

摘要

研究了植物生长调节剂（PGRs）和外植体类型对油橄榄（Olea europaea L. ssp europaea var. sativa）品种“Chetoui”胚发生和植株再生的影响，采用未成熟合子胚进行研究。在添加低浓度不同BAP和NAA的OMc培养基上实现了胚性愈伤组织诱导。未成熟合子胚作为幼嫩组织，不依赖PGRs补充即可进行体细胞胚发生。在无PGRs培养基上或黑暗中低浓度条件下可获得重复体细胞胚发生。