Muhammad Neak, Le Han Ho, Lee Yong-Jae, Ko Jaeho, Nguyen Tra T H, Kim Song-Gun
Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup, Jeonbuk 56212, Republic of Korea.
University of Science and Technology (UST), 217 Gajeong-ro, Yuseong, Daejeon 34113, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005458.
A Gram-stain-negative and rod-shaped bacterial strain (WSW3-B6) was isolated from red alga collected from the West Sea, Republic of Korea. Cells of strain WSW3-B6 were non-motile, aerobic and produced slightly yellow and mucoid colonies on marine agar. The strain grew optimally at 23-30 °C, with 0.5-4 % NaCl (w/v) and at pH 6.5-8.5. A phylogenetic analysis of the 16S rRNA gene revealed that strain WSW3-B6 belongs to the genus within the family , having the highest sequence similarity to SM1502 (96.7%), followed by subsp. LaA7.5 (96.2%) and subsp. SaA2.12 (96.2%). The complete sequence of a circular chromosome of strain WSW3-B6 determined by combination of Oxford Nanopore and Illumina platforms comprised a total 2 725 095 bp with G+C content of 37.1 mol%. A comparative analysis based on the whole genome also showed the distinctiveness of strain WSW3-B6. The average nucleotide identity (ANI) values between strain WSW3-B6 and the closest strains SM1502, subsp. LaA7.5 and subsp. SaA2.12 were 78.3, 77.8 and 77.7 %, respectively, while the digital DNA-DNA hybridization (dDDH) values between strain WSW3-B6 and the above closely related strains were 21.0, 20.4 and 20.3 %, respectively. Both the ANI and dDDH values supported the creation of a new species in the genus . The major fatty acids (>10 %) were iso-C (19.3 %), C (14.0 %), iso-C 3-OH (13.1 %) and C (10.7 %). The polar lipids of strain WSW3-B6 included phosphatidylethanolamine, three unidentified aminolipids and three unidentified lipids. Moreover, MK-6 was the only respiratory quinone. A comparison of the phylogenetic distinctiveness and the unique phenotypic and chemotaxonomic characteristics among strain WSW3-B6 and closely related type strains supported that strain WSW3-B6 (=KCTC 82708=GDMCC 1.2627) represents a novel species of the genus , for which the name sp. nov. is proposed.
从韩国西海采集的红藻中分离出一株革兰氏阴性杆状细菌菌株（WSW3-B6）。WSW3-B6菌株的细胞无运动性，好氧，在海洋琼脂上产生浅黄色黏液状菌落。该菌株在23-30 °C、0.5-4 %（w/v）NaCl和pH 6.5-8.5条件下生长最佳。对16S rRNA基因的系统发育分析表明，WSW3-B6菌株属于 科内的 属，与 SM1502的序列相似性最高（96.7%），其次是 亚种 LaA7.5（96.2%）和 亚种 SaA2.12（96.2%）。通过牛津纳米孔和Illumina平台相结合确定的WSW3-B6菌株环状染色体的完整序列全长2 725 095 bp，G+C含量为37.1 mol%。基于全基因组的比较分析也显示了WSW3-B6菌株的独特性。WSW3-B6菌株与最接近的菌株 SM1502、 亚种 LaA7.5和 亚种 SaA2.12之间的平均核苷酸同一性（ANI）值分别为78.3%、77.8%和77.7 %，而WSW3-B6菌株与上述密切相关菌株之间的数字DNA-DNA杂交（dDDH）值分别为21.0%、20.4%和20.3 %。ANI和dDDH值均支持在 属中创建一个新物种。主要脂肪酸（>10 %）为异-C （19.3 %）、C （14.0 %）、异-C 3-OH（13.1 %）和C （10.7 %）。WSW3-B6菌株的极性脂质包括磷脂酰乙醇胺、三种未鉴定的氨基脂质和三种未鉴定的脂质。此外，MK-6是唯一的呼吸醌。对WSW3-B6菌株与密切相关模式菌株之间的系统发育独特性以及独特的表型和化学分类特征进行比较，支持WSW3-B6菌株（=KCTC 82708=GDMCC 1.2627）代表 属的一个新物种，为此提出新物种名称 sp. nov.。
