Debnath Sanjit Chandra, Chen Can, Khan Ishrat, Wang Wen-Jie, Zheng Dao-Qiong, Xu Jin-Zhong, Wang Pin-Mei
Ocean College, Zhejiang University, Zhoushan, PR China.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4250-4260. doi: 10.1099/ijsem.0.004281.
Two yellow-pigmented, Gram-stain-negative, aerobic, rod-shaped bacteria were isolated from the water of the hypersaline Chaka Salt Lake (strain SaA2.12) and sediment of Qinghai Lake (strain LaA7.5), PR China. According to the 16S rRNA phylogeny, the isolates belong to the genus , showing the highest 16S rRNA sequence similarities to SM1502(97.6-97.7 %) and XIN-1(96.5-96.6 %). Moreover, strains SaA2.12 and LaA7.5 showed 99.73 % 16S rRNA sequence similarity to each other. Major fatty acids, respiratory quinones and polar lipids detected in these isolates were iso-C, menaquinone-6 and phosphatidylethanolamine, respectively. Strains SaA2.12 and LaA7.5 showed significant unique characteristics between them as well as between the closest phylogenetic members. The highest digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SaA2.12 and its closest neighbours were 25.3 and 82.8 %, respectively; whereas these values (highest) between LaA7.5 and its closest members were 25.2 and 82.8 %, respectively. The dDDH and ANI values between strains SaA2.12 and LaA7.5 were calculated as 75.9 and 97.2 %, respectively. Therefore, based on polyphasic data, we propose that strain SaA2.12 represents a novel species with the name sp. nov., with the type strain SaA2.12 (=KCTC 72220=MCCC 1K03618) and strain LaA7.5 as a subspecies within novel with the name subsp. subsp. nov., with the type strain LaA7.5 (=KCTC 72806=MCCC 1K04372). These propositions automatically create subsp. subsp. nov. with SaA2.12 (=KCTC 72220=MCCC 1K03618) as the type strain.
从中国青海察尔汗盐湖的水体（菌株SaA2.12）和青海湖的沉积物（菌株LaA7.5）中分离出两株黄色色素沉着、革兰氏染色阴性、需氧的杆状细菌。根据16S rRNA系统发育分析，这些分离株属于 属，与SM1502（97.6 - 97.7%）和XIN - 1（96.5 - 96.6%）显示出最高的16S rRNA序列相似性。此外，菌株SaA2.12和LaA7.5彼此之间的16S rRNA序列相似性为99.73%。在这些分离株中检测到的主要脂肪酸、呼吸醌和极性脂质分别为异C、甲基萘醌 - 6和磷脂酰乙醇胺。菌株SaA2.12和LaA7.5在它们自身之间以及与最接近的系统发育成员之间都表现出显著的独特特征。SaA2.12与其最接近的邻居之间的最高数字DNA - DNA杂交（dDDH）和平均核苷酸同一性（ANI）值分别为25.3%和82.8%；而LaA7.5与其最接近成员之间的这些值（最高）分别为25.2%和82.8%。菌株SaA2.12和LaA7.5之间的dDDH和ANI值分别计算为75.9%和97.2%。因此，基于多相数据，我们提议菌株SaA2.12代表一个新物种，命名为 新种，模式菌株为SaA2.12（=KCTC 72220 = MCCC 1K03618），菌株LaA7.5作为新 属内的一个亚种，命名为 亚种 新亚种，模式菌株为LaA7.5（=KCTC 72806 = MCCC 1K04372）。这些提议自动创建了以SaA2.12（=KCTC 72220 = MCCC 1K03618）为模式菌株的 亚种 新亚种。
