Nedashkovskaya Olga I, Kim Song-Gun, Stenkova Anna M, Kukhlevskiy Andrey D, Zhukova Natalia V, Mikhailov Valery V
G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Mar;68(3):892-898. doi: 10.1099/ijsem.0.002606. Epub 2018 Jan 29.
A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-orange pigmented flavobacterium, designated strain 9Alg 151, was isolated from the Pacific red alga Tichocarpus crinitus. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain fell into the genus Aquimarina of the family Flavobacteriaceae with a 16S rRNA gene sequence similarity range of 94.2-98.2 % to the recognized species of the genus. Strain 9Alg 151 grew in the presence of 0.5-5 % NaCl and at 5-34 °C, and hydrolysed aesculin, agar, gelatin, starch, Tween 40, DNA and chitin. The predominant fatty acids were iso-C17 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C16 : 0, iso-C17 : 1ω8c and summed feature 3. The polar lipid profile comprised phosphatidylethanolamine, three unidentified aminolipids and three unidentified lipids. The major respiratory quinone was MK-6. The genomic DNA G+C content was 32.6 mol%. On the basis of 16S rRNA gene sequence data, and chemotaxonomic and phenotypic characteristics, strain 9Alg 151 represents a novel species of the genus Aquimarina, for which the name Aquimarina algiphila sp. nov. is proposed. The type strain is 9Alg 151 (=KCTC 23622=KMM 6462).
从太平洋红藻繁枝蜈蚣藻中分离出了一株严格需氧、革兰氏染色阴性、杆状、靠滑动运动且呈黄橙色色素的黄杆菌，命名为菌株9Alg 151。基于16S rRNA基因序列的系统发育分析表明，该新菌株属于黄杆菌科的海栖菌属，其16S rRNA基因序列与该属已确认物种的相似性范围为94.2 - 98.2%。菌株9Alg 151在0.5 - 5% NaCl存在的条件下以及5 - 34°C生长，并能水解七叶苷、琼脂、明胶、淀粉、吐温40、DNA和几丁质。主要脂肪酸为异-C17 : 0 3-OH、异-C15 : 0、异-C15 : 1 G、异-C15 : 0 3-OH、异-C16 : 0、异-C17 : 1ω8c和特征性总和3。极性脂质谱包含磷脂酰乙醇胺、三种未鉴定的氨基脂质和三种未鉴定的脂质。主要呼吸醌为MK-6。基因组DNA G+C含量为32.6 mol%。基于16S rRNA基因序列数据以及化学分类和表型特征，菌株9Alg 151代表海栖菌属的一个新物种，为此提出名为嗜藻海栖菌（Aquimarina algiphila sp. nov.）。模式菌株为9Alg 151（=KCTC 23622=KMM 6462）。
