Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, Modern Separation Science Key Laboratory of Shaanxi Province, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, PR China.
Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, Modern Separation Science Key Laboratory of Shaanxi Province, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Oct 1;1208:123408. doi: 10.1016/j.jchromb.2022.123408. Epub 2022 Aug 6.
In this work, we prepared a novel graphene-based composite by combination of mercapto-end capped polyhedral oligomeric silsesquioxane (POSS-SH) with graphene oxide (GO) through covalent bonding process. The composite was further conjugated with 4-vinyl pyridine (4-VP) through thiol-ene click reaction to fabricate pseudo-mercaptoethyl pyridine functionalized POSS-graphene composite (G-POSS-S-VP). The physicochemical properties were confirmed via FT-IR, TGA, XPS, and TEM characterizations and a high ligand density of ca. 87.3 μmol m was obtained. G-POSS-S-VP composite displayed high selectivity toward antibody, i.e., immunoglobulin G (IgG) in this case based on the hydrophobic charge induced chromatography (HCIC) mechanism. A high adsorption efficiency of ca. 90% and large adsorption capacity of 500 mg g were achieved for IgG at pH 7.4. At the same time, the non-specific adsorptions of co-existing proteins on graphene substrate were obviously hindered. The practical applicability of G-POSS-S-VP composite as adsorbent was verified by selective separation of IgG from human serum. SDS-PAGE assay and MALDI-TOF-MS analysis clearly demonstrated the successful isolation of IgG with high purity.
在这项工作中，我们通过共价键过程将巯基封端的多面体低聚倍半硅氧烷（POSS-SH）与氧化石墨烯（GO）结合，制备了一种新型的基于石墨烯的复合材料。然后，通过硫醇-烯点击反应将该复合材料与 4-乙烯基吡啶（4-VP）进一步键合，制备了假巯基乙基吡啶功能化 POSS-石墨烯复合材料（G-POSS-S-VP）。通过傅里叶变换红外光谱（FT-IR）、热重分析（TGA）、X 射线光电子能谱（XPS）和透射电子显微镜（TEM）等手段对其物理化学性质进行了确认，并得到了约 87.3 μmol/m 的高配体密度。G-POSS-S-VP 复合材料基于疏水电荷诱导色谱（HCIC）机制，对抗体（此处为免疫球蛋白 G（IgG））表现出很高的选择性。在 pH 7.4 时，IgG 的吸附效率约为 90%，吸附容量高达 500mg/g。同时，明显抑制了共存在石墨烯基底上的蛋白质的非特异性吸附。通过从人血清中选择性分离 IgG 验证了 G-POSS-S-VP 复合材料作为吸附剂的实际应用。SDS-PAGE 分析和 MALDI-TOF-MS 分析清楚地表明 IgG 得到了高纯度的分离。
