College of Environmental Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, China.
College of Environmental Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, China; Shanghai institute of pollution control and ecological security, Shanghai 200092, China.
Bioresour Technol. 2022 Oct;361:127754. doi: 10.1016/j.biortech.2022.127754. Epub 2022 Aug 9.
In this study, the combination of dielectric barrier discharge plasma (DBD) with calcium peroxide (CaO) achieved significant synergistic effects in promoting hydrolysis of waste activated sludge (WAS) and short-chain fatty acid (SCFA) production during anaerobic fermentation. Compared with the control, DBD/CaO pretreatment increased SCFA production by 116 %, acetic acid ratio by 39 %, and sludge reduction by 30 % under the optimal conditions (discharge power = 76.5 W, CaO dosage = 0.05 g/g VSS). Mechanism investigations elucidated that DBD/CaO enhanced the generation of •OH, O, and •O, synergistically promoted decomposing extracellular polymeric substances (EPS), lysing cells, releasing biodegradable substances, and enhancing acetic acid-enriched SCFA accumulation from fermentation. Meanwhile, Illumina MiSeq sequencing analysis revealed that the enrichment of hydrolytic and SCFAs-forming bacteria and the decrease in SCFAs-consuming bacteria by DBD/CaO treatment also contributed. This work provides an effective method to boost the SCFA production from WAS fermentation.
在这项研究中，介质阻挡放电等离子体（DBD）与过氧化钙（CaO）的结合在促进厌氧发酵过程中废活性污泥（WAS）水解和短链脂肪酸（SCFA）生产方面取得了显著的协同效应。与对照相比，在最佳条件下（放电功率=76.5 W，CaO 用量=0.05 g/g VSS），DBD/CaO 预处理使 SCFA 产量增加了 116%，乙酸比例增加了 39%，污泥减少了 30%。机理研究表明，DBD/CaO 增强了•OH、O 和•O 的生成，协同促进了胞外聚合物（EPS）的分解、细胞裂解、可生物降解物质的释放，并增强了发酵过程中乙酸富集的 SCFA 积累。同时，Illumina MiSeq 测序分析表明，DBD/CaO 处理对水解和 SCFA 形成菌的富集以及对 SCFA 消耗菌的减少也有贡献。这项工作为从 WAS 发酵中提高 SCFA 产量提供了一种有效的方法。
