College of Environmental Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, China.
College of Environmental Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; National Circular Economy Engineering Laboratory, Shanghai 201620, China.
Bioresour Technol. 2023 Nov;387:129618. doi: 10.1016/j.biortech.2023.129618. Epub 2023 Aug 5.
Suitable waste activated sludge (WAS) pretreatments that boost short-chain fatty acid (SCFA) production from anaerobic fermentation are essential for carbon emission reduction and sludge resource utilization. This study established an efficient WAS pretreatment process combining atmospheric pressure plasma jet (APPJ) with peracetic acid (PAA). The maximum SCFA production (6.5-fold that of the control) largely increased under the optimal conditions (PAA dosage = 25 mg/g VSS (volatile suspended solids), energy consumption = 20.9 kWh/m). APPJ/PAA pretreatment enhanced the production of multiple reactive species (including OH, CHC(O)O, O, ONOO, O, and e) and strengthened the effects of HO, heat, and light. This synergistically solubilized WAS and released organic substrates for SCFA-producing microbes. In addition, the enrichment of SCFA-producing bacteria and the decrease in SCFA-consuming bacteria favored SCFA accumulation. The key genes encoding for the main substrate metabolism and SCFA production in the metabolic pathway of fermentation were also enhanced.
合适的废活性污泥（WAS）预处理方法可促进厌氧发酵中短链脂肪酸（SCFA）的生成，这对于减少碳排放和污泥资源利用至关重要。本研究建立了一种将大气压等离子射流（APPJ）与过氧乙酸（PAA）相结合的高效 WAS 预处理工艺。在最佳条件下（PAA 用量为 25mg/gVSS（挥发性悬浮固体），能耗为 20.9kWh/m），SCFA 的最大产量（是对照的 6.5 倍）大幅增加。APPJ/PAA 预处理增强了多种活性物质（包括 OH、CHC(O)O、O、ONOO、O 和 e）的产生，并强化了 HO、热和光的作用。这协同作用使 WAS 溶解，并释放有机底物供 SCFA 产生微生物利用。此外，SCFA 产生菌的富集和 SCFA 消耗菌的减少有利于 SCFA 的积累。发酵代谢途径中主要底物代谢和 SCFA 生成的关键基因也得到了增强。
